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α-Parvin and β-parvin in the rat uterus during decidualisation and uterine receptivity

  • Leigh Nicholson
  • Romanthi Madawala
  • Laura Lindsay
  • Christopher R. Murphy
Original Paper
  • 11 Downloads

Abstract

During early pregnancy, the uterine luminal epithelial cells (UECs) and endometrial stromal cells (ESCs) undergo morphological changes to enable blastocyst implantation. The present study investigates, for the first time, the cytoskeletal-associated proteins and α-actinin superfamily members, α-parvin and β-parvin, during early pregnancy in the rat uterus. These two PARVA proteins are involved in cell adhesion, morphological changes and regulation of other cytoskeletal proteins, through binding with proteins such as actin and integrin-linked kinase. α-parvin is present in UECs at fertilisation and significantly decreases by the time of implantation. β-parvin acts in opposition; significantly increasing in both UECs and ESCs at the time of implantation, suggesting a role in the process of decidualisation. Additionally, the presence of a serine-8 residue-phosphorylated α-parvin, which is associated with cell morphology changes, was found in the nuclear region of both UECs and ESCs during implantation and decidualisation. We also show that the presence of both β-parvin and phosphorylated α-parvin in ESCs is dependent on decidualisation occurring. This study demonstrates that the changing balance and localisation of the two PARVA proteins are dependent on the time of uterine receptivity, suggesting a co-dependent role in the cytoskeletal re-organisation crucial to the changing conditions necessary for implantation and decidualisation.

Keywords

Endometrium Decidualisation Early pregnancy Cytoskeleton Parvin 

Notes

Acknowledgements

The support and facilities of the Bosch Institute Molecular Biology Facility and the Bosch Institute Advanced Microscopy Facility, The University of Sydney.

Funding

Financial support was provided by the Australian Research Council, The Ann Macintosh Foundation of the Discipline of Anatomy and Histology and the Murphy Laboratory.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Cell and Reproductive Biology Lab, Discipline of Anatomy and Histology, School of Medical SciencesUniversity of SydneyCamperdownAustralia
  2. 2.Kolling Institute of Medical ResearchRoyal North Shore HospitalSt LeonardsAustralia

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