Histochemistry and Cell Biology

, Volume 144, Issue 3, pp 273–279 | Cite as

Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor

  • Dong-Joon Lee
  • Chengri Jin
  • Eun-Jung Kim
  • Jong-Min Lee
  • Han-Sung Jung
Original Paper


Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide’s receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.


Gastrin-releasing peptide (GRP) Incisor development Ameloblast Odontoblast Dentin Enamel 



This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute(KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C3266). This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number : HI14C1817).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS ProjectYonsei University College of DentistrySeoulKorea
  2. 2.Department of DentistryAffiliated Hospital of Yanbian UniversityYanjiChina
  3. 3.Department of Oral Biosciences, Faculty of DentistryThe University of Hong KongHong Kong SARChina

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