Histochemistry and Cell Biology

, Volume 142, Issue 4, pp 389–399 | Cite as

Inhibition of JNK in HaCaT cells induced tight junction formation with decreased expression of cytokeratin 5, cytokeratin 17 and desmoglein 3

  • Norio Kitagawa
  • Yuko Inai
  • Yoshinori Higuchi
  • Hiroshi Iida
  • Tetsuichiro Inai
Original Paper
First Online:
Accepted:

Abstract

Epidermal keratinocytes proliferate in the basal layer, differentiate, migrate through the spinous layer, granular layer and cornified layer, and finally are peeled off from the surface of skin with layer-specific expression of differentiation markers, including cytokeratins and cell–cell junction proteins such as desmogleins. Basal cells express CK5, CK14 and Ki67. In contrast, the suprabasal cells in the spinous and granular layers express CK1 and CK10 without Ki67. Inhibition of c-Jun NH2-terminal protein kinase (JNK) in HaCaT cells, a human epidermal keratinocyte cell line, induced the formation of tight junctions, which occurs in the granular layer in vivo. These cells lost their expression of CK5 and CK17, exhibited decreased expression of desmoglein 3 and had no Ki67 labeling in the nucleus. These results suggest that inhibition of JNK causes HaCaT cells to differentiate from basal- and spinous-like cells to granular-like cells. The inhibition of JNK in HaCaT cells provides a useful in vitro model system to study the differentiation of epidermal keratinocytes.

Keywords

Mitogen-activated protein kinase (MAPK) c-Jun NH2-terminal protein kinase (JNK) Tight junction Cytokeratin Desmoglein HaCaT cell 
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Notes

Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research (C) (No. 23590249) and the MEXT-Supported Program for the Strategic Research Foundation at Private Universities (S1001059), 2010–2015 from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Norio Kitagawa
    • 1
  • Yuko Inai
    • 2
  • Yoshinori Higuchi
    • 2
  • Hiroshi Iida
    • 3
  • Tetsuichiro Inai
    • 1
  1. 1.Department of Morphological BiologyFukuoka Dental CollegeFukuokaJapan
  2. 2.Dental Section, Division of General Oral CareKyushu University HospitalFukuokaJapan
  3. 3.Laboratory of Zoology, Graduate School of AgricultureKyushu UniversityFukuokaJapan

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