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Histochemistry and Cell Biology

, Volume 141, Issue 6, pp 577–585 | Cite as

The changing point-spread function: single-molecule-based super-resolution imaging

  • Mathew H. Horrocks
  • Matthieu Palayret
  • David Klenerman
  • Steven F. Lee
Review

Abstract

Over the past decade, many techniques for imaging systems at a resolution greater than the diffraction limit have been developed. These methods have allowed systems previously inaccessible to fluorescence microscopy to be studied and biological problems to be solved in the condensed phase. This brief review explains the basic principles of super-resolution imaging in both two and three dimensions, summarizes recent developments, and gives examples of how these techniques have been used to study complex biological systems.

Keywords

Single-molecule microscopy Super-resolution imaging PALM/(d)STORM imaging Localization microscopy 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Mathew H. Horrocks
    • 1
  • Matthieu Palayret
    • 1
  • David Klenerman
    • 1
  • Steven F. Lee
    • 1
  1. 1.Department of ChemistryUniversity of CambridgeCambridgeUK

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