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Histochemistry and Cell Biology

, Volume 141, Issue 5, pp 499–506 | Cite as

Expression of adenosine A2b receptor in rat type II and III taste cells

  • Kentaro Nishida
  • Yukari Dohi
  • Yuri Yamanaka
  • Ai Miyata
  • Katsunobu Tsukamoto
  • Miharu Yabu
  • Akihiro Ohishi
  • Kazuki Nagasawa
Original Paper

Abstract

We previously demonstrated that equilibrative nucleoside transporter 1 was expressed in taste cells, suggesting the existence of an adenosine signaling system, but whether or not the expression of an adenosine receptor occurs in rat taste buds remains unknown. Therefore, we examined the expression profiles of adenosine receptors and evaluated their functionality in rat circumvallate papillae. Among adenosine receptors, the mRNA for an adenosine A2b receptor (A2bR) was expressed by the rat circumvallate papillae, and its expression level was significantly greater in the circumvallate papillae than in the non-taste lingual epithelium. A2bR-immunoreactivity was detected primarily in type II taste cells, and partial, but significant expression was also observed in type III ones, but there was no immunoreactivity in type I ones. The cAMP generation in isolated epithelium containing taste buds treated with 500 μM adenosine or 10 μM BAY60-6583 was significantly increased compared to in the controls. These findings suggest that adenosine plays a role in signaling transmission via A2bR between taste cells in rats.

Keywords

Adenosine A2b receptor Taste cell Circumvallate papillae 

Abbreviations

ATP

Adenosine 5′-triphosphate

ADP

Adenosine 5′-diphosphate

AMP

Adenosine 5′-monophosphate

ENT

Equilibrative nucleoside transporter

GAD

Glutamic acid decarboxylase

IBMX

3-Isobutyl-1-methylxanthine

IP3R3

Inositol 1,4,5-trisphosphate receptor, type 3

NTPDase

Nucleoside triphosphate diphosphohydrolase

5-HT

Serotonin

Notes

Acknowledgments

Parts of this study were financially supported by a Grant-in-Aid for Young Scientists (B) (22790134) from the Ministry of Education, Science and Culture of Japan, the ‘Academic Frontier’ Project for Private Universities of the Japanese Ministry of Education, Culture, Sport, Science and Technology, and the Kyoto Pharmaceutical University Fund for the Promotion of Scientific Research.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Kentaro Nishida
    • 1
  • Yukari Dohi
    • 1
  • Yuri Yamanaka
    • 1
  • Ai Miyata
    • 1
  • Katsunobu Tsukamoto
    • 1
  • Miharu Yabu
    • 1
  • Akihiro Ohishi
    • 1
  • Kazuki Nagasawa
    • 1
  1. 1.Department of Environmental BiochemistryKyoto Pharmaceutical UniversityYamashina-ku, KyotoJapan

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