First evidence of the interaction between deleted in malignant brain tumor 1 and galectin-3 in the mammalian oviduct
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The oviduct supports the transport and final maturation of gametes, and harbors fertilization and early embryo development. The oviductal epithelium is responsible for providing the correct environment for these processes. Deleted in malignant brain tumor 1 (DMBT1) is expressed by multiple organisms and several cell types, and the interaction of the rabbit ortholog of DMBT1 with galectin-3 (gal-3) modulates the polarity of epithelial cells. This interaction has not yet been shown in locations other than rabbit kidney and human-cultured endothelial cells. DMBT1 and gal-3 also protect epithelial layers from pathogens and trauma, and are innate immunity components. DMBT1 has been detected in the porcine oviduct, and gal-3 has been reported in the Fallopian tube and in the cow oviduct. Interaction between both proteins would show a probable physiological function in the female reproductive tract. This work describes the presence and co-localization of DMBT1 and gal-3 mainly in the apical region of the epithelial cells of the Fallopian tube and the porcine oviduct, and co-immunoprecipitation in membrane-enriched epithelial cell extracts from the porcine oviduct. The findings strongly support a functional interaction in the mammalian oviduct, suggestive of a role on epithelial protection and homeostasis, which might be related to epithelium–gamete interaction.
KeywordsOviduct Fallopian tube DMBT1 Galectin-3
This study was supported by Agencia Nacional de Promoción de la Ciencia y Tecnología—BID program (Grant number PICT 1284-2007) of Argentina, and Grant 21010-098-12 from Secretaría de Estado de Ciencia, Tecnología e Innovación de la Provincia de Santa Fe, Argentina. We thank Dr. Fu-Tong Liu for anti-galectin-3 antibodies, Dr. Jan Mollenhauer for anti-DMBT1p84 antibodies, and Dr. Sergio Ghersevich for Fallopian tube samples. We also thank Frigorífico Paladini SA for providing gilt’s oviducts.
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