RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells
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RBM8A (Y14) is carrying RNA-binding motif and forms the tight heterodimer with MAGOH. The heterodimer is known to be a member of exon junction complex on exporting mRNA and is required for mRNA metabolisms such as splicing, mRNA export and nonsense-mediated mRNA decay. Almost all RBM8A–MAGOH complexes localize in nucleoplasm and shuttle between nuclei and cytoplasm for RNA metabolism. Recently, the abnormality of G2/M transition and aberrant centrosome regulation in RBM8A- or MAGOH-deficient cells has been reported. These results prompt us to the reevaluation of the localization of RBM8A–MAGOH in human cells. Interestingly, our immunostaining experiments showed the localization of these proteins in centrosome in addition to nuclei. Furthermore, the transiently expressed eYFP-tagged RBM8A and Flag-tagged MAGOH also co-localized with centrosome signals. In addition, the proximity ligation in situ assay was performed to detect the complex formation in centrosome. Our experiments clearly showed that Myc-tagged RBM8A and Flag-tagged MAGOH formed a complex in centrosome. GFP-tagged PLK1 also co-localized with Myc-RBM8A. Our results show that RBM8A–MAGOH complex is required for M-phase progression via direct localization to centrosome rather than indirect effect.
KeywordsRBM8A (Y14) MAGOH Cell cycle Centrosome
Authors also thank to Prof. Tsutomu Takegami, Prof. Hideaki Nakagawa and Ms. Yoshie Yoshida at the Kanazawa Medical University for their kind support. This work was supported by Grants from Kanazawa Medical University (S2012-1, SR2012-02), Sumitomo Science Foundation, Grants of Strategic Research Project (H2012-16[S1201022]) from Kanazawa Medical University and Ministry of Education, Culture, Sports, Science and Technology, Japan and Grant-in-Aid for Scientific Research in Japan (KAKENHI #25460376).
Conflict of interest
The authors have read the journal’s policy and have the following conflicts: Prof. Naohisa Tomosugi is the president of Medical Care Proteomics Biotechnology Co., Ltd. This does not alter the author’s adherence to all the policies on sharing data.
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