Histochemistry and Cell Biology

, Volume 139, Issue 3, pp 403–413 | Cite as

Expression of ezrin in subventricular zone neural stem cells and their progeny in adult and developing mice

  • Younghye Moon
  • Joo Yeon Kim
  • So Yoen Choi
  • Hyo Min Cho
  • Hyun Kim
  • Woong Sun
Original Paper

Abstract

Ezrin is a member of the ezrin–radixin–moesin (ERM) family of proteins, which link the cytoskeleton and cell membrane. ERM proteins are involved in pivotal cellular functions including cell–matrix recognition, cell–cell communication, and cell motility. Several recent studies have shown that ERM proteins are expressed in specific cell types of the adult rostral migratory stream (RMS). In this study, we found that ERM proteins are expressed highly in the early postnatal RMS and the ventricular zone of embryonic cerebral cortex, suggesting that these proteins may be expressed by neural progenitors. Furthermore, whereas ezrin previously was found to be expressed exclusively by astrocytes of the adult RMS, we found that ezrin-expressing cells also expressed the markers for indicating neuroblasts in vivo and in vitro, and that ezrin expression by neuroblasts decreases progressively as neuroblasts migrate. Using in vitro differentiation of adult neural stem cells, we found that ezrin is expressed by neural stem cells and their progeny (neuroblasts and astrocytes), but not by oligodendrocytic progeny. Collectively our findings demonstrate that adult neural stem cells and neuroblasts express ezrin and that ezrin may be involved in intracellular actin remodeling.

Keywords

Ezrin ERM protein Neuroblast Rostral migratory stream Neural stem cell 

Abbreviations

SVZ

Subventricular zone

OB

Olfactory bulb

RMS

Rostral migratory stream

ERM

Ezrin–radixin–moesin

CNS

Central nervous system

VZ

Ventricular zone

DG

Dentate gyrus

Notes

Acknowledgments

This work was supported by grants from the Korean Ministry of Education, Science and Technology, via the Brain Research Center of the 21st century Frontier Program in Neuroscience (2012K001108) and the National Research Foundation of Korea (20120006433, 20120005815, 2012049933).

Supplementary material

418_2012_1048_MOESM1_ESM.doc (29 kb)
Supplementary material 1 (DOC 29 kb)
418_2012_1048_MOESM2_ESM.jpg (1.7 mb)
Supplementary material 2 (JPG 1.70 mb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Younghye Moon
    • 1
  • Joo Yeon Kim
    • 1
  • So Yoen Choi
    • 1
  • Hyo Min Cho
    • 1
  • Hyun Kim
    • 1
  • Woong Sun
    • 1
  1. 1.Department of Anatomy and Division of Brain Korea 21 Biomedical ScienceKorea University College of MedicineSeoulKorea

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