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Histochemistry and Cell Biology

, Volume 136, Issue 6, pp 677–688 | Cite as

Dictyostelium puromycin-sensitive aminopeptidase A is a nucleoplasmic nucleomorphin-binding protein that relocates to the cytoplasm during mitosis

  • Andrew Catalano
  • Yekaterina Poloz
  • Danton H. O’Day
Original Paper

Abstract

Nucleomorphin (NumA1) is a nucleolar/nucleoplasmic protein linked to cell cycle in Dictyostelium. It interacts with puromycin-sensitive aminopeptidase A (PsaA) which in other organisms is a Zn2+-metallopeptidase thought to be involved in cell cycle progression and is involved in several human diseases. Here, we have shown that Dictyostelium PsaA contains domains characteristic of the M1 family of Zn2+-metallopeptidases: a GAMEN motif and a Zn2+-binding domain. PsaA colocalized with NumA1 in the nucleoplasm in vegetative cells and was also present to a lesser extent in the cytoplasm. The same localization pattern was observed in cells from slugs, however, in fruiting bodies PsaA was only detected in spore nuclei. During mitosis PsaA redistributed mainly throughout the cytoplasm. It possesses a functional nuclear localization signal (680RKRF683) necessary for nuclear entry. To our knowledge, this is the first nuclear localization signal identified in a Psa from any organism. Treatment with Ca2+ chelators or calmodulin antagonists indicated that neither Ca2+ nor calmodulin is involved in PsaA localization. These results are interpreted in terms of the inter-relationship between NumA1 and PsaA in cell function in Dictyostelium.

Keywords

Puromycin-sensitive aminopeptidase Nucleoplasm Mitosis Nuclear localization signal Nucleomorphin 

Notes

Acknowledgments

This work was supported by the Natural Sciences and Engineering Research Council of Canada (DHO’D; A6807).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Andrew Catalano
    • 1
  • Yekaterina Poloz
    • 1
  • Danton H. O’Day
    • 1
    • 2
  1. 1.Department of Cell and Systems BiologyUniversity of TorontoTorontoCanada
  2. 2.Department of BiologyUniversity of Toronto at MississaugaMississaugaCanada

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