Lectins for histochemical demonstration of glycans
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Lectins have been proven to be invaluable reagents for the histochemical detection of glycans in cells and tissues by light and electron microscopy. This technical review deals with the conditions of tissue fixation and embedding for lectin labeling, as well as various markers and related labeling techniques. Furthermore, protocols for lectin labeling of sections from paraffin and resin-embedded tissues are detailed together with various controls to demonstrate the specificity of the labeling by lectins.
KeywordsLectins Histochemistry Light microscopy Electron microscopy Colloidal gold Digoxigenin Silver amplification
I wish to express my gratitude to former coworkers Pierre M. Charest, John M. Lucocq, Tetsutaro Sata, Douglas J. Taatjes, Valeriu Toma and Christian Zuber as well as Irwin J. Goldstein for fruitful collaboration. I thank Paul Debbage for critical reading of the manuscript. Supported by the World Class University program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (R31-2008-000-10086-0) and a grant from the National Research Foundation of Korea by the Ministry of Education, Science and Technology (2010-0027736). The Swiss National Science Foundation supported the original work of the author between 1983 and 2009.
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