Histochemistry and Cell Biology

, Volume 135, Issue 3, pp 281–292 | Cite as

Leptin and vascular endothelial growth factor regulate angiogenesis in tooth germs

  • Shinji Ide
  • Reiko Tokuyama
  • Purevsuren Davaadorj
  • Masashi Shimozuma
  • Shuku Kumasaka
  • Seiko Tatehara
  • Kazuhito SatomuraEmail author
Original Paper


Leptin, a 16 kDa non-glycolated polypeptide of 146 amino acids produced by the ob gene, has a variety of physiological roles not only in lipid metabolism, hematopoiesis, thermogenesis and ovarian function, but also in angiogenesis. This study focuses to investigate the possibility that leptin, as an angiogenic factor, may regulate the angiogenesis during tooth development. We firstly studied the expression of leptin and vascular endothelial growth factor (VEGF) during tooth development immunohistochemically. This investigation revealed that leptin is expressed in ameloblasts, odontoblasts, dental papilla cells and stratum intermedium cells. This expression pattern was similar to that of VEGF, one of the most potent angiogenic factors. Interestingly, more leptin-positive cells were observed in the upper third portion of dental papilla, which is closest to odontoblastic layer, compared to middle and lower thirds. Moreover, in the dental papilla, more CD31 and/or CD34-positive vascular endothelial cells were observed in the vicinity of ameloblasts and odontoblasts expressing leptin and VEGF. These findings strongly suggest that ameloblasts, odontoblasts and dental papilla cells induce the angiogenesis in tooth germs by secretion of leptin as well as VEGF.


Leptin VEGF CD31 CD34 Tooth development Angiogenesis 



This work was supported by a Grant-in-Aid for Scientific Research (21592575, 22791986 and 22592233) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Shinji Ide
    • 1
  • Reiko Tokuyama
    • 1
  • Purevsuren Davaadorj
    • 2
  • Masashi Shimozuma
    • 1
  • Shuku Kumasaka
    • 1
  • Seiko Tatehara
    • 1
  • Kazuhito Satomura
    • 1
    Email author
  1. 1.Second Department of Oral and Maxillofacial Surgery, Department of Oral Medicine and Stomatology, School of Dental MedicineTsurumi UniversityYokohamaJapan
  2. 2.Department of Oral and Maxillofacial Surgery, Institute of Health BiosciencesThe University of Tokushima Graduate SchoolTokushimaJapan

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