Histochemistry and Cell Biology

, Volume 134, Issue 6, pp 603–614 | Cite as

Possible involvement of maspin in tooth development

  • Purevsuren Davaadorj
  • Reiko Tokuyama
  • Shinji Ide
  • Susumu Tadokoro
  • Keiko Kudoh
  • Kazuhito Satomura
Original Paper
First Online:
Accepted:

Abstract

Maspin is a 42 kDa serine protease inhibitor that possesses tumor suppressive and anti-angiogenic activities. Despite of a huge amount of data concerning the expression pattern of maspin in various tissues and its relevance to the biological properties of a variety of human cancer cells, little is known on the maspin expression in skeletal and tooth tissues. Recently, we reported that maspin may play an important role in extracellular matrix formation in bone by enhancing the accumulation of latent TGF-β in the extracellular matrix. This study was performed to elucidate the possible role of maspin in tooth development. First, an immunohistochemical analysis for human tooth germs at the late bell stage showed the expression of maspin by active ameloblasts and odontoblasts that were forming enamel and dentin, respectively. During rat tooth development, maspin expression was observed for the first time in inner and outer enamel epithelial cells and dental papilla cells at early bell stage. The neutralizing anti-maspin antibody inhibited the proper dental tissue formation in organ cultures of mandibular first molars obtained from 21-day-old rat embryos. In addition, the proliferation of HAT-7 cells, a rat odontogenic epithelial cell line, and human dental papilla cells were suppressed in a dose-dependent manner with anti-maspin antibody. Moreover, RT-PCR analysis showed that the expression of mRNA for tooth-related genes including dentin matrix protein 1, dentin sialophosphoprotein and osteopontin in human dental papilla cells was inhibited when treated with anti-maspin antibody. These findings suggest that maspin expressed in ameloblasts and odontoblasts plays an important physiological role in tooth development through the regulation of matrix formation in dental tissues.

Keywords

Maspin Serine protease inhibitor Tooth development Ameloblast Odontoblast Extracellular matrix 
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Notes

Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research (19390518, 21592575 and 20380090) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors wish to thank Dr. Masayuki Azuma and Dr. Keiko Miyoshi (Institute of Health Bioscience, The University of Tokushima Graduate School) for critical reading of the manuscript.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Purevsuren Davaadorj
    • 1
  • Reiko Tokuyama
    • 3
  • Shinji Ide
    • 3
  • Susumu Tadokoro
    • 3
  • Keiko Kudoh
    • 2
  • Kazuhito Satomura
    • 3
  1. 1.Department of Oral and Maxillofacial Surgery, Institute of Health BiosciencesThe University of Tokushima Graduate SchoolTokushimaJapan
  2. 2.Department of Oral Surgery, Institute of Health BiosciencesThe University of Tokushima Graduate SchoolTokushimaJapan
  3. 3.Second Department of Oral and Maxillofacial Surgery, Department of Oral Medicine and Stomatology, School of Dental MedicineTsurumi UniversityKanagawaJapan

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