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Histochemistry and Cell Biology

, Volume 134, Issue 2, pp 137–144 | Cite as

Expression and localization of an agmatinase-like protein in the rat brain

  • Claudia Mella
  • Fernando Martínez
  • María de los Angeles García
  • Francisco Nualart
  • Víctor Castro
  • Paulina Bustos
  • Nelson Carvajal
  • Elena Uribe
Original Paper

Abstract

Agmatinase catalyzes the hydrolysis of agmatine into putrescine and urea, and agmatine (decarboxylated l-arginine) plays several roles in mammalian tissues, including neurotransmitter/neuromodulatory actions in the brain. Injection of agmatine in animals produces anticonvulsant, antineurotoxic and antidepressant-like actions. Information regarding the enzymatic aspects of agmatine metabolism in mammals, especially related to its degradation, is relatively scarce. The explanation for this is the lack of enzymatically active preparations of mammalian agmatinase. Recently, we have cloned a protein from a cDNA rat brain library having agmatinase activity although its amino acid sequence greatly differs from all known agmatinases, we called agmatinase-like protein. In this work, we analyzed the expression of this enzyme in the rat brain by means of RT-PCR and immunohistochemical analysis using a polyclonal antibody generated against the recombinant agmatinase-like protein. The agmatinase-like protein was detected in the hypothalamus in glial cells and arcuate nucleus neurons, and in hippocampus astrocytes and neurons, but not in brain cortex. In general, detected localization of agmatinase-like protein coincides with that described for its substrate agmatine and our results help to explain several reported effects of agmatine in the brain. Concretely, a role in the regulation of intracellular concentrations of the neurotransmitter/neuromodulator agmatine is suggested for the brain agmatinase-like protein.

Keywords

Agmatinase Agmatine Hypothalamus Astrocytes 

Notes

Acknowledgments

This research was supported by Grant Fondecyt 11070069 and ACT02.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Claudia Mella
    • 1
  • Fernando Martínez
    • 2
  • María de los Angeles García
    • 2
  • Francisco Nualart
    • 2
  • Víctor Castro
    • 1
  • Paulina Bustos
    • 3
  • Nelson Carvajal
    • 1
  • Elena Uribe
    • 1
  1. 1.Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias BiológicasUniversidad de ConcepciónConcepciónChile
  2. 2.Departamento de Biología Celular, Facultad de Ciencias BiológicasUniversidad de ConcepciónConcepciónChile
  3. 3.Departamento de Bioquímica Clínica, Facultad de FarmaciaUniversidad de ConcepciónConcepciónChile

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