Tissue stretch induces nuclear remodeling in connective tissue fibroblasts
- 363 Downloads
Studies in cultured cells have shown that nuclear shape is an important factor influencing nuclear function, and that mechanical forces applied to the cell can directly affect nuclear shape. In a previous study, we demonstrated that stretching of whole mouse subcutaneous tissue causes dynamic cytoskeletal remodeling with perinuclear redistribution of α-actin in fibroblasts within the tissue. We have further shown that the nuclei of these fibroblasts have deep invaginations containing α-actin. In the current study, we hypothesized that tissue stretch would cause nuclear remodeling with a reduced amount of nuclear invagination, measurable as a change in nuclear concavity. Subcutaneous areolar connective tissue samples were excised from 28 mice and randomized to either tissue stretch or no stretch for 30 min, then examined with histochemistry and confocal microscopy. In stretched tissue (vs. non-stretched), fibroblast nuclei had a larger cross-sectional area (P < 0.001), smaller thickness (P < 0.03) in the plane of the tissue, and smaller relative concavity (P < 0.005) indicating an increase in nuclear convexity. The stretch-induced loss of invaginations may have important influences on gene expression, RNA trafficking and/or cell differentiation.
KeywordsCytoskeleton Subcutaneous Nucleus Mechanotransduction Invagination
The authors thank Drs. Nicholas Heintz and William C. Eanrshaw for helpful discussions. This work was funded by the National Institutes of Health Center for Complementary and Alternative Medicine research Grant RO1-AT01121 and by National Institutes of Health Grant P20 RR16435 from the Center of Biomedical Research Excellence Program of the National Center for Research Resources. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Center for Complementary and Alternative Medicine, National Institutes of Health.
Conflict of interest statement
Helene M. Langevin is a partner of Stromatec, Inc.
- de Berg M (1997) Computational geometry: algorithms and applications. Springer, BerlinGoogle Scholar
- Gieni RS, Hendzel MJ (2007) Mechanotransduction from the ECM to the genome: are the pieces now in place? J Cell Biochem 104:1964–1987Google Scholar
- Horn B (1986) Robot vision. MIT Press/McGraw-Hill, CambridgeGoogle Scholar
- Pratt WK (1991) Digital image processing. Wiley, NY, pp 636–644Google Scholar