Prostasin inhibits cell invasion in human choriocarcinomal JEG-3 cells
- 205 Downloads
Controlled invasion of the uterine wall by the trophoblast cells is pivotal for the successful pregnancy, and various kinds of protease are involved in this process. Serine protease prostasin has been shown to participate in the proteolytic activation of epithelial sodium channel as well as cleavage of epidermal growth factor receptor extracellular domain in human epithelial cells. Its physiological significance in human placentation has been suggested but not validated. In the present study, we found that prostasin was expressed at a relatively high level in human placenta trophoblasts in early pregnant weeks. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4. Our data indicated that this serine protease may function as an invasion suppressor in human trophoblast, participating in the invasion-restrictive regulation of trophoblasts to avoid their over-penetration into the uterine wall.
KeywordsProstasin Cell invasion Choriocarcinomal JEG-3 cell MMPs/TIMPs Integrins
The work was supported by the grants from National Natural Sciences Foundation (No. 30530760), the Chinese National Special Fund for Basic Research Project (No. 2006CB944008) and the Knowledge Innovation Program in Chinese Academy of Sciences (No. KSCX2-YW-R-53). The authors appreciate the kind gift of MMP-26 and TIMP-4 antibodies from Dr. Qing-xiang Amy Sang at Florida State University).
- Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity. Cancer Res 47:943–946 Google Scholar