Histochemistry and Cell Biology

, Volume 132, Issue 6, pp 639–646 | Cite as

Prostasin inhibits cell invasion in human choriocarcinomal JEG-3 cells

  • Xiao-jie Ma
  • Ya-yuan Fu
  • Yu-xia Li
  • Li-mei Chen
  • Karl Chai
  • Yan-ling Wang
Original Paper

Abstract

Controlled invasion of the uterine wall by the trophoblast cells is pivotal for the successful pregnancy, and various kinds of protease are involved in this process. Serine protease prostasin has been shown to participate in the proteolytic activation of epithelial sodium channel as well as cleavage of epidermal growth factor receptor extracellular domain in human epithelial cells. Its physiological significance in human placentation has been suggested but not validated. In the present study, we found that prostasin was expressed at a relatively high level in human placenta trophoblasts in early pregnant weeks. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4. Our data indicated that this serine protease may function as an invasion suppressor in human trophoblast, participating in the invasion-restrictive regulation of trophoblasts to avoid their over-penetration into the uterine wall.

Keywords

Prostasin Cell invasion Choriocarcinomal JEG-3 cell MMPs/TIMPs Integrins 

Notes

Acknowledgments

The work was supported by the grants from National Natural Sciences Foundation (No. 30530760), the Chinese National Special Fund for Basic Research Project (No. 2006CB944008) and the Knowledge Innovation Program in Chinese Academy of Sciences (No. KSCX2-YW-R-53). The authors appreciate the kind gift of MMP-26 and TIMP-4 antibodies from Dr. Qing-xiang Amy Sang at Florida State University).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Xiao-jie Ma
    • 1
    • 3
  • Ya-yuan Fu
    • 1
    • 3
  • Yu-xia Li
    • 1
  • Li-mei Chen
    • 2
  • Karl Chai
    • 2
  • Yan-ling Wang
    • 1
  1. 1.State Key Laboratory of Reproductive Biology, Institute of ZoologyChinese Academy of SciencesBeijingChina
  2. 2.Department of Molecular Biology and MicrobiologyUniversity of Central FloridaOrlandoUSA
  3. 3.Graduate School of Chinese Academy of SciencesBeijingChina

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