Histochemistry and Cell Biology

, Volume 133, Issue 1, pp 125–134 | Cite as

Characterization of newly established bovine intestinal epithelial cell line

  • Kohtaro Miyazawa
  • Tetsuya Hondo
  • Takashi Kanaya
  • Sachi Tanaka
  • Ikuro Takakura
  • Wataru Itani
  • Michael T. Rose
  • Haruki Kitazawa
  • Takahiro Yamaguchi
  • Hisashi AsoEmail author
Original Paper


Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer’s patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.


Bovine small intestine Epithelial cell line Cell differentiation M cells Peyer’s patches 



This research was supported by a Grant-in-Aid for Scientific Research (21380170) from the Ministry of Education, Culture, Sports, Science and Technology, BSE Control Project from the Ministry of Agriculture, Forestry and Fisheries, and Postdoctoral Fellowships for Research Abroad from Japan Society for the Promotion of Science (JSPS).


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Kohtaro Miyazawa
    • 1
    • 2
  • Tetsuya Hondo
    • 1
  • Takashi Kanaya
    • 1
  • Sachi Tanaka
    • 1
  • Ikuro Takakura
    • 1
  • Wataru Itani
    • 1
  • Michael T. Rose
    • 3
  • Haruki Kitazawa
    • 4
  • Takahiro Yamaguchi
    • 1
  • Hisashi Aso
    • 1
    Email author
  1. 1.Laboratory of Functional Morphology, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan
  2. 2.Section of Neuropathology, Department of SurgeryYale Medical SchoolNew HavenUSA
  3. 3.Institute of Biological, Environmental and Rural SciencesAberystwyth UniversityCardiganshireUK
  4. 4.Laboratory of Animal Products Chemistry, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan

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