Characterization of newly established bovine intestinal epithelial cell line
- 931 Downloads
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer’s patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.
KeywordsBovine small intestine Epithelial cell line Cell differentiation M cells Peyer’s patches
This research was supported by a Grant-in-Aid for Scientific Research (21380170) from the Ministry of Education, Culture, Sports, Science and Technology, BSE Control Project from the Ministry of Agriculture, Forestry and Fisheries, and Postdoctoral Fellowships for Research Abroad from Japan Society for the Promotion of Science (JSPS).
- Borghesi C, Taussig MJ, Nicoletti C (1999) Rapid appearance of M cells after microbial challenge is restricted at the periphery of the follicle-associated epithelium of Peyer’s patch. Lab Invest J Tech Methods Pathol 79:1393–1401Google Scholar
- Kanaya T, Miyazawa K, Takakura I, Itani W, Watanabe K, Ohwada S, Kitazawa H, Rose MT, McConochie HR, Okano H, Yamaguchi T, Aso H (2008) Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. Am J Physiol 295:G273–G284Google Scholar
- Schierack P, Nordhoff M, Pollmann M, Weyrauch KD, Amasheh S, Lodemann U, Jores J, Tachu B, Kleta S, Blikslager A, Tedin K, Wieler LH (2006) Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine. Histochem Cell Biol 125:293–305CrossRefPubMedGoogle Scholar
- Weng XH, Beyenbach KW, Quaroni A (2005) Cultured monolayers of the dog jejunum with the structural and functional properties resembling the normal epithelium. Am J Physiol 288:G705–G717Google Scholar