Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers

  • Erdal KaraozEmail author
  • Ayça Aksoy
  • Selda Ayhan
  • Ayla Eker Sarıboyacı
  • Figen Kaymaz
  • Murat Kasap
Original Paper


Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated that BM-MSCs expressed myogenic (desmin, myogenin, myosin IIa, and α-SMA), neurogenic (γ-enolase, MAP2a,b, c-fos, nestin, GFAP and beta III tubulin), and osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, BMP-2, BMP-4 and type I collagen) markers without stimulation towards differentiation. These expression patterns indicated why these cells can easily differentiate into multiple lineages both in vitro and in vivo. Ultrastructural characteristics of rBM-MSCs showed more developed and metabolically active cells.


Rat Bone marrow Stem cell Differentiation Proliferation Ultrastructure Immunophenotype 



This study was supported by grant (107S276) of the Scientific and Research Council of Turkey (TUBITAK). We thank Alparslan Okcu and Gulcin Gacar for their excellent technical assistance.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Erdal Karaoz
    • 1
    Email author
  • Ayça Aksoy
    • 1
  • Selda Ayhan
    • 1
  • Ayla Eker Sarıboyacı
    • 1
  • Figen Kaymaz
    • 2
  • Murat Kasap
    • 1
  1. 1.Stem Cell and Gene Therapy Research and Application CenterKocaeli UniversityKocaeliTurkey
  2. 2.Department of Histology and Embryology, Faculty of MedicineHacettepe UniversityAnkaraTurkey

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