Expression of nestin, desmin and vimentin in intact and regenerating muscle spindles of rat hind limb skeletal muscles
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We describe the expression and distribution patterns of nestin, desmin and vimentin in intact and regenerating muscle spindles of the rat hind limb skeletal muscles. Regeneration was induced by intramuscular isotransplantation of extensor digitorum longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. The host muscles with grafts were excised after 7-, 16-, 21- and 29-day survival and immunohistochemically stained. Nestin expression in intact spindles in host muscles was restricted to Schwann cells of sensory and motor nerves. In transplanted muscles, however, nestin expression was also found in regenerating “spindle fibers”, 7 and 16 days after grafting. From the 21st day onwards, the regenerated spindle fibers were devoid of nestin immunoreactivity. Desmin was detected in spindle fibers at all developmental stages in regenerating as well as in intact spindles. Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting. Our results show that the expression pattern of these intermediate filaments in regenerating spindle fibers corresponds to that found in regenerating extrafusal fibers, which supports our earlier suggestion that they resemble small-diameter extrafusal fibers.
KeywordsIntermediate filaments Muscle spindle regeneration Skeletal muscle transplantation Rat muscle spindles Rat hind limb
The authors thank Mrs. Helena Rückerová, Mrs. Hana Hollerová, Mrs. Magda Kudrnová and Ms. Jana Kudrnáčová for their skillful technical assistance. This work was supported by the following grant projects: 304/08/0329 and 304/08/0256 from the Grant Agency of the Czech Republic, MSM0021620820 from the Ministry of Education of the Czech Republic, MYORES LSH-CT-2004-511978 and by the Research project AV0Z 50110509.
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