A long-term study on female mice fed on a genetically modified soybean: effects on liver ageing
Liver represents a suitable model for monitoring the effects of a diet, due to its key role in controlling the whole metabolism. Although no direct evidence has been reported so far that genetically modified (GM) food may affect health, previous studies on hepatocytes from young female mice fed on GM soybean demonstrated nuclear modifications involving transcription and splicing pathways. In this study, the effects of this diet were studied on liver of old female mice in order to elucidate possible interference with ageing. The morpho-functional characteristics of the liver of 24-month-old mice, fed from weaning on control or GM soybean, were investigated by combining a proteomic approach with ultrastructural, morphometrical and immunoelectron microscopical analyses. Several proteins belonging to hepatocyte metabolism, stress response, calcium signalling and mitochondria were differentially expressed in GM-fed mice, indicating a more marked expression of senescence markers in comparison to controls. Moreover, hepatocytes of GM-fed mice showed mitochondrial and nuclear modifications indicative of reduced metabolic rate. This study demonstrates that GM soybean intake can influence some liver features during ageing and, although the mechanisms remain unknown, underlines the importance to investigate the long-term consequences of GM-diets and the potential synergistic effects with ageing, xenobiotics and/or stress conditions.
KeywordsAgeing Cell nucleus Genetically modified soybean Liver Mitochondria
We thank M. Storaci of the Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche for his assistance in mouse breeding. This work was supported by the Agenzia Servizi Settore Agroalimentare delle Marche, Italy.
- Kuiper HA, König A, Kleter GA, Hammes WP, Knudsen I, European Network on Safety Assessment of Genetically Modified Food Crops (ENTRANSFOOD) (2004) Safety assessment, detection and traceability, and societal aspects of genetically modified foods. European Network on Safety Assessment of Genetically Modified Food Crops (ENTRANSFOOD). Food Chem Toxicol 42:1195–1202PubMedCrossRefGoogle Scholar
- Padgette SR, Kolacz KH, Delannay X, Re DB, LaVallee BJ, Tinius CN, Rhodes WK, Otero YI, Barry GF, Eichholtz DA, Peschke VM, Nida DL, Taylor NB, Kishore GM (1995) Development, identification and characterization of a glyphosate-tolerant soybean line. Crop Sci 35:1451–1461Google Scholar
- Pastorelli R, Carpi D, Campagna R, Airoldi L, Pohjanvirta R, Viluksela M, Hakansson H, Boutros PC, Moffat ID, Okey AB, Fanelli R (2006) Differential expression profiling of the hepatic proteome in a rat model of dioxin resistance: correlation with genomic and transcriptomic analyses. Mol Cell Proteomics 5:882–894PubMedCrossRefGoogle Scholar
- Trabalza-Marinucci M, Brandi G, Rondini C, Avellini L, Giammarini C, Costarelli S, Acuti G, Orlandi C, Filippini G, Chiaradia E, Malatesta M, Crotti S, Antonini C, Amagliani G, Manuali E, Mastrogiacomo AR, Moscati L, Haouet MN, Gaiti A, Magnani M (2008) A three year longitudinal study on the effects of a diet containing genetically modified Bt176 maize on the health status and performance on sheep. Livestock Sci 113:178–190CrossRefGoogle Scholar
- Vecchio L, Cisterna B, Malatesta M, Martin TE, Biggiogera M (2004) Ultrastructural analysis of testes from mice fed on genetically modified soybean. Eur J Histochem 48:449–453Google Scholar