Differential expression pattern of protein ARVCF in nephron segments of human and mouse kidney
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The protein ARVCF is a member of the p120 subfamily of armadillo proteins whose members have been described to occur in junction-bound and non-junction-bound forms. Studies on ARVCF were constrained because the endogenous protein was difficult to detect with the available reagents. We have generated novel monoclonal and polyclonal antibodies usable for biochemical and localization studies. By systematic immunohistochemical analysis of various tissues protein ARVCF is prominently detected in mouse, bovine and human kidney. Using antibodies against specific markers of nephron segments protein ARVCF is localized in proximal tubules according to double label immunofluorescence. Besides its occurrence in proximal tubules of adult kidney and in renal cell carcinoma derived from proximal tubules ARVCF is also detected in maturing nephrons in early mouse developmental stages such as, for example, 15 days of gestation (E15). Immunoblotting of total extracts of cultured cells of renal origin showed that ARVCF is detected in all human and murine cultured cells analyzed. Upon immunolocalization ARVCF is mostly detected in the cytoplasm occurring in a fine granular form. This prominent cytoplasmic localization of ARVCF in cultured cells and its occurrence in proximal tubules implies an involvement of ARVCF in specific functional processes of proximal tubules of kidney.
KeywordsArm-repeat protein p120-catenin subfamily Nephron Proximal tubule Renal cell carcinoma
We thank C. Kuhn (Division of Cell Biology, German Cancer Research Center) for experimental help in the initial course of this study and A. Wiss (Department of Pathology, University of Heidelberg) for competent help in sectioning paraffin-embedded human tissues. For continuous and generous support Prof. Dr. W. W. Franke is thanked (Division of Cell Biology, German Cancer Research Center). We gratefully acknowledge the critical comments of Dr. M. Elger (Institute of Anatomy, University Heidelberg) and Dr. B. Falkowska-Hansen (Genetics of Skin Carcinogenesis, German Cancer Research Center). This study was supported by a project grant from the German Ministry for Education and Research (BMBF) in a cooperative research program entitled “Standardization of mesenchymal stem cells for regenerative medicine (START-MSC)”.
- Borrmann CM, Grund C, Kuhn C, Hofmann I, Pieperhoff S, Franke WW (2006) The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Occurrence of both desmosomal and fascia adhaerens molecules in the intercalated disks. Eur J Cell Biol 85:469–485PubMedCrossRefGoogle Scholar
- Hofmann I, Casella M, Schnölzer M, Schlechter T, Spring H, Franke WW (2006) Identification of the junctional plaque protein plakophilin 3 in cytoplasmic particles containing RNA-binding proteins and the recruitment of plakophilins 1 and 3 to stress granules. Mol Biol Cell 17:1388–1398PubMedCrossRefGoogle Scholar
- Kaufman MH (1995) The atlas of mouse development. Revised 2nd edn. Academic Press Limited, LondonGoogle Scholar
- Piepenhagen PA, Nelson WJ (1998) Biogenesis of polarized epithelial cells during kidney development in situ: roles of E-cadherin-mediated cell–cell adhesion and membrane cytoskeleton organization. Mol Biol Cell 11:3161–3177Google Scholar
- Sirotkin H, O’Donnell H, DasGupta R, Halford S, St Jore B, Puech A, Parimoo S, Morrow S, Skoultchi A, Weissman SM, Scambler P, Kucherlapati R (1997) Identification of a new human catenin gene family member (ARVCF) from the region deleted in velo-cardio-facial syndrome. Genomics 41:75–83PubMedCrossRefGoogle Scholar