Histochemistry and Cell Biology

, Volume 130, Issue 5, pp 943–956 | Cite as

Differential expression pattern of protein ARVCF in nephron segments of human and mouse kidney

  • Britta Walter
  • Tanja Schlechter
  • Michaela Hergt
  • Irina Berger
  • Ilse HofmannEmail author
Original Paper


The protein ARVCF is a member of the p120 subfamily of armadillo proteins whose members have been described to occur in junction-bound and non-junction-bound forms. Studies on ARVCF were constrained because the endogenous protein was difficult to detect with the available reagents. We have generated novel monoclonal and polyclonal antibodies usable for biochemical and localization studies. By systematic immunohistochemical analysis of various tissues protein ARVCF is prominently detected in mouse, bovine and human kidney. Using antibodies against specific markers of nephron segments protein ARVCF is localized in proximal tubules according to double label immunofluorescence. Besides its occurrence in proximal tubules of adult kidney and in renal cell carcinoma derived from proximal tubules ARVCF is also detected in maturing nephrons in early mouse developmental stages such as, for example, 15 days of gestation (E15). Immunoblotting of total extracts of cultured cells of renal origin showed that ARVCF is detected in all human and murine cultured cells analyzed. Upon immunolocalization ARVCF is mostly detected in the cytoplasm occurring in a fine granular form. This prominent cytoplasmic localization of ARVCF in cultured cells and its occurrence in proximal tubules implies an involvement of ARVCF in specific functional processes of proximal tubules of kidney.


Arm-repeat protein p120-catenin subfamily Nephron Proximal tubule Renal cell carcinoma 



We thank C. Kuhn (Division of Cell Biology, German Cancer Research Center) for experimental help in the initial course of this study and A. Wiss (Department of Pathology, University of Heidelberg) for competent help in sectioning paraffin-embedded human tissues. For continuous and generous support Prof. Dr. W. W. Franke is thanked (Division of Cell Biology, German Cancer Research Center). We gratefully acknowledge the critical comments of Dr. M. Elger (Institute of Anatomy, University Heidelberg) and Dr. B. Falkowska-Hansen (Genetics of Skin Carcinogenesis, German Cancer Research Center). This study was supported by a project grant from the German Ministry for Education and Research (BMBF) in a cooperative research program entitled “Standardization of mesenchymal stem cells for regenerative medicine (START-MSC)”.

Supplementary material

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Britta Walter
    • 1
    • 3
  • Tanja Schlechter
    • 1
    • 2
  • Michaela Hergt
    • 1
  • Irina Berger
    • 3
  • Ilse Hofmann
    • 1
    • 4
    Email author
  1. 1.Division of Cell BiologyGerman Cancer Research CenterHeidelbergGermany
  2. 2.Progen BiotechnikHeidelbergGermany
  3. 3.Institute of PathologyUniversity of HeidelbergHeidelbergGermany
  4. 4.Joint Research Division Vascular Biology of the Medical Faculty MannheimUniversity of Heidelberg and the German Cancer Research Center, CBTMMannheimGermany

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