Histochemistry and Cell Biology

, Volume 130, Issue 2, pp 375–385

Promoter-defined isolation and identification of hepatic progenitor cells from the human fetal liver

Original Paper

DOI: 10.1007/s00418-008-0439-2

Cite this article as:
Wang, P., Zhang, H., Li, W. et al. Histochem Cell Biol (2008) 130: 375. doi:10.1007/s00418-008-0439-2

Abstract

Hepatoblasts, which are considered one type of hepatic progenitor cell, reside in the fetal liver. To selectively identify these cells, we transfected primary cultured human fetal liver cells (FLCs) with a pGL3 vector bearing the gene for the enhanced green fluorescence protein (EGFP) under the control of the alpha-fetoprotein (AFP) promoter expressed in hepatoblasts. The FLCs were then sorted by fluorescence-activated cell sorting (FACS) on the basis of AFP promoter-driven EGFP expression. The EGFP-positive cells expressed AFP, albumin, and cytokeratin 19, and could be expanded in vitro. Thus, the AFP promoter-EGFP reporter system is highly useful for identification and isolation of hepatic progenitor cells.

Keywords

Hepatic progenitor cells Alpha-fetoprotein Promoter Human fetus Identification 

Abbreviations

AFP

Alpha-fetoprotein

ALB

Albumin

CK19

Cytokeratin 19

EGFP

Enhanced green fluorescence protein

FACS

Fluorescence-activated cell sorting

FLCs

Fetal liver cells

HSCs

Hematopoietic stem cells

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of RegenerationCapital Medical UniversityBeijingChina
  2. 2.Key Laboratory for Neurodegenerative Diseases of Ministry of EducationXuanwu Hospital, Capital Medical UniversityBeijingChina

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