Ultrastructural localization and chemical binding of silver ions in human organotypic skin cultures
- 141 Downloads
Organotypic cultures of human breast skin incubated with silver bandage or treated with silver sulfadiazine accumulated silver in epithelial cells and in macrophages, fibroblasts and collagen fibrils and fibres of underlying connective tissue. Ultrastructurally, the accumulated silver was found in lysosome-like vesicles of the different cells and evenly spread along collagen structures. Apoptotic nuclei were present but few. Autometallographic amplification of 2D-PAGE gels revealed that glutathione S-transferase and glutathion detoxify silver ions in the epidermal cell by binding them in silver–sulphur nanocrystals. Thus, the cytotoxic effect of silver ions seems to be muted by silver ions by being: (1) taken up by undamaged cells, neutralised by glutathione (GSH) and accumulated in lysosomal vesicles, (2) bound extracellularly to SH-groups of the collagen fibres.
KeywordsSilver Wound healing Glutathione S-transferase Autometallography Multiple resistance proteins Gluthatione
- Danscher G, Stoltenberg M (2006) Silver enhancement of quantum dots resulting from (1) metabolism of toxic metals in animals and humans (2) in vivo, in vitro and immersion created zinc-sulphur/zinc-selenium nanocrystals, (3) metal ions liberated from metal implants and particles. Prog Histochem Cytochem 41:57–139PubMedCrossRefGoogle Scholar
- Jorgensen B, Price P, Andersen KE, Gottrup F, Bech-Thomsen N, Scanlon E, Kirsner R, Rheinen H, Roed-Petersen J, Romanelli M, Jemec G, Leaper DJ, Neumann MH, Veraart J, Coerper S, Agerslev RH, Bendz SH, Larsen JR, Sibbald RG (2005) The silver-releasing foam dressing, contreet foam, promotes faster healing of critically colonised venous leg ulcers: a randomised, controlled trial. Int Wound J 2:64–73PubMedCrossRefGoogle Scholar
- Lansdown AB (2006) Silver in health care: antimicrobial effects and safety in use. Curr Probl Dermatol 33:17–34:17–34Google Scholar
- Rungby J, Danscher G (1984) Hypoactivity in silver exposed mice. Acta Pharmacol Toxicol (Copenh) 55:398–401Google Scholar