Histochemistry and Cell Biology

, Volume 128, Issue 4, pp 323–333

Expression of the leptin receptor in different types of vascular lesions

  • Marco R. Schroeter
  • Jacob Schneiderman
  • Bettina Schumann
  • Roland Glückermann
  • Petros Grimmas
  • Arnd B. Buchwald
  • Theodor Tirilomis
  • Friedrich A. Schöndube
  • Stavros V. Konstantinides
  • Katrin Schäfer
Original Paper

Abstract

Clinical and experimental evidence suggests that the adipokine leptin may be important for the development of cardiovascular complications associated with obesity, possibly through interaction with its receptor on vascular cells. In the present study, we systematically analysed expression of the leptin receptor in normal and diseased vascular specimens using immunohistochemistry, immunofluorescence and quantitative real time-PCR. In particular, human atherosclerotic plaques as well as experimental vascular lesions induced in hypercholesterolemic mice and minipigs, respectively, were examined. Our results demonstrate the presence of the leptin receptor in normal vessel wall segments as well as neointimal or atherosclerotic lesions. In the latter, ObR expressing cells were predominantly localised on the luminal border and within the subintima, and coexpression of von Willebrand factor, VEGF receptor-2 or VE cadherin identified them as endothelial cells. Moreover, CD14-positive monocytes/macrophages were strongly positive for the leptin receptor. In contrast, only few ObR-expressing smooth muscle cells could be detected in human atherosclerotic plaques. The findings of the present study thus support a possible action of leptin on the cardiovascular system by demonstrating expression of the leptin receptor in different types of vascular lesions.

Keywords

Atherosclerosis Fluorescence microscopy Leptin receptor Neointima Obesity 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Marco R. Schroeter
    • 1
  • Jacob Schneiderman
    • 2
  • Bettina Schumann
    • 1
  • Roland Glückermann
    • 1
  • Petros Grimmas
    • 1
  • Arnd B. Buchwald
    • 3
  • Theodor Tirilomis
    • 4
  • Friedrich A. Schöndube
    • 4
  • Stavros V. Konstantinides
    • 1
  • Katrin Schäfer
    • 1
    • 5
  1. 1.Department of Cardiology and Pulmonary MedicineGeorg August University of GöttingenGöttingenGermany
  2. 2.Department of Vascular Surgery and the Gottesdiener Vascular Biology Laboratory, The Chaim Sheba Medical Center, Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael
  3. 3.Städtisches Krankenhaus Kiel, I. Medizinische KlinikKielGermany
  4. 4.Department of Cardiothoracic and Vascular SurgeryUniversity of GöttingenGöttingenGermany
  5. 5.Department of Cardiology and Pulmonary MedicineUniversity of GöttingenGöttingenGermany

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