Histochemistry and Cell Biology

, Volume 127, Issue 4, pp 423–432 | Cite as

Expression of androgen receptor and its co-localization with estrogen receptor-alpha in the developing pituitary gland of sheep fetus

  • XueJun Yuan
  • YuQin He
  • JiaLi Liu
  • HaoShu Luo
  • JinHua Zhang
  • Sheng Cui
Original Paper

Abstract

No information is known concerning the expression of androgen receptor (AR) and its co-localization with estrogen receptor alpha (ERα) in the developing pituitary of sheep fetus. In the present study, we detected AR expression and its co-localization with ERα in the anterior pituitary of sheep fetus from day 60 of gestation to the postnatal by dual immunochemistry. The results showed that both AR immunoreactivity (AR-ir) and ERα immunoreactivity (ERα-ir) were predominantly localized in the nuclei of LH positive gonadotropes. The cell counting results showed that the percentage of the anterior pituitary cells expressing AR fluctuated from 13.51 ± 0.92 to 17.05 ± 1.83% during the examined stages, but there were no significant differences between sexes and among ages examined (P > 0.05). However, the proportion of AR-ir cells containing LH markedly increased from day 60 of gestation to the neonatal (P < 0.05). The percentage of AR-ir cells expressing ERα-ir significantly increased from day 60 of gestation to the neonatal, respectively (P < 0.05), but no significant differences were seen between genders at each stage examined. These results indicate that both AR and ERα are mainly expressed in the gonadotropes of anterior pituitary gland of sheep fetuses, whereas the functions and interaction of AR and ERα expressions in the developing pituitary gland are required to be elucidated further.

Keywords

Immunohistochemistry AR ERα Sheep fetus Pituitary 

Notes

Acknowledgments

This work was supported from grants of the Natural Science Foundation of Beijing (6042013) and the Natural Science Foundation of China (30325034, 30471264). We thank Prof. AS McNeilly (Centre for reproductive biology, Edinburgh, UK) for the kind gift of rabbit anti-ovine LH and PRL antibody. We also thank Professor GL Xia, Department of Animal Physiology, College of Biological Sciences, China Agricultural University, People’s Republic of China for reviewing the English manuscript. Anti-ovine GH, anti-ovine TSH and anti-human ACTH primary antibodies were obtained through the National Hormone and Peptide Program (NHPP), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) & Dr Parlow (NHPP).

Supplementary material

418_2006_262_MOESM1_ESM.doc (520 kb)
ESM1 (DOC 520 kb)

References

  1. Beato M, Herrlich P, Schutz G (1995) Steroid hormone receptors: many actors in search of a plot. Cell 83:851–857PubMedCrossRefGoogle Scholar
  2. Brandenberger AW, Tee MK, Lee JY, Chao V, Jaffe RB (1997) Tissue distribution of estrogen receptors alpha (ER-alpha) and beta (ER-beta) mRNA in the midgestational human fetus. J Clin Endocrinol Metab 82:3509–3512PubMedCrossRefGoogle Scholar
  3. Choate JV, Slayden OD, Resko JA (1998) Immunocytochemical localization of androgen receptors in brains of developing and adult male rhesus monkeys. Endocrine 8:51–60PubMedCrossRefGoogle Scholar
  4. Clarke IJ (2002) Multifarious effects of estrogen on the pituitary gonadotrope with special emphasis on studies in the ovine species. Arch Physiol Biochem 110:62–73PubMedCrossRefGoogle Scholar
  5. Crocoll A, Zhu CC, Cato AC, Blum M (1998) Expression of androgen receptor mRNA during mouse embryogenesis. Mech Dev 72:175–178PubMedCrossRefGoogle Scholar
  6. Cui S, Liu JL, ShaoYJ, Zhang JC (2004) Parallel changes between the percentage of fetal pituitary cells immunoreactive to oestrogen receptor α and the concentration of 17β-oestradiol in fetal and maternal plasma during gestation in sheep. Reprod Fertil Dev 16:611–616PubMedCrossRefGoogle Scholar
  7. Gonzalez-Parra S, Argente J, Garcia-Segura LM, Chowen JA (1998) Cellular composition of the adult rat anterior pituitary is influenced by the neonatal sex steroid environment. Neuroendocrinology 68:152–162PubMedCrossRefGoogle Scholar
  8. Gonzazalez-Parra S, Argente J, Garcia-Segura LM, Chowen JA (2000) Effect of neonatal and adult testosterone treatment on the cellular composition of the adult female rat anterior pituitary. J Endocrinol 164:265–276PubMedCrossRefGoogle Scholar
  9. Goyal HO, Bartol FF, Wiley AA, Khalil MK, Chiu J, Vig MM (1997) Immunolocalization of androgen receptor and estrogen receptor in the developing testis and excurrent ducts of goats. Anat Rec 249:54–62PubMedCrossRefGoogle Scholar
  10. Handa RJ, Stadelman HL, Resko JA (1987) Effect of estrogen on androgen receptor dynamics in female rat pituitary. Endocrinology 121:84–89PubMedCrossRefGoogle Scholar
  11. Hasui K, Takatsuka T, Sakamoto R, Matsushita S, Tsuyama S, Izumo S, Murata F (2003) Double autoimmunostaining with glycine treatment. J Histochem Cytochem 51:1169–1176PubMedGoogle Scholar
  12. Hileman SM, Lubbers LS, Kuehl DE, Schaeffer DJ, Rhodes L, Jackson GL (1994) Effect of inhibiting 5-alpha-reductase activity on the ability of testosterone to inhibit luteinizing hormone release in male sheep. Biol Reprod 50:1244–1250PubMedCrossRefGoogle Scholar
  13. Iqbal J, Swanson JJ, Prins GS, Jacobson CD (1995) Androgen receptor-like immunoreactivity in the Brazilian opossum brain and pituitary: distribution and effects of castration and testosterone replacement in the adult male. Brain Res 703:1–18PubMedCrossRefGoogle Scholar
  14. Jorgensen JS, Nilson JH (2001) AR suppresses transcription of the LH beta subunit by interacting with steroidogenicfactor-1. Mol Endocrinol 15:1505–1516PubMedCrossRefGoogle Scholar
  15. Keller ET, Ershler WB, Chang C (1996) The androgen receptor: a mediator of diverse responses. Front Biosci 1:d59–d71PubMedGoogle Scholar
  16. Lindzey J, Wetsel WC, Couse JF, Stoker T, Cooper R, Korach KS (1998) Effects of Castration and chronic steroid treatments on hypothalamic gonadotropin-releasing hormone content and pituitary gonadotropins in male wild-type and estrogen receptor-α knockout mice. Endocrinology 139:4092–4101PubMedCrossRefGoogle Scholar
  17. Liu J, Cui S (2005) Ontogeny of estrogen receptor (ER) alpha and its co-localization with pituitary hormones in the pituitary gland of chick embryos. Cell Tissue Res 320:235–242PubMedCrossRefGoogle Scholar
  18. MacLusky N, Naftolin F (1981) Sexual differentiation of the central nervous system. Science 211:1294–1302PubMedCrossRefGoogle Scholar
  19. McCann JP, Mayes JS, Hendricks GR, Harjo JB, Watson GH (2001) Subcellular distribution and glycosylation pattern of androgen receptor from sheep omental adipose tissue. J Endocrinol 169:587–593PubMedCrossRefGoogle Scholar
  20. Mesiano S, Hart CS, Heyer BW, Kaplan SL, Grumbach MM (1991) Hormone ontogeny in the ovine fetus. XXVI. A sex difference in the effect of castration on the hypothalamic-pituitary gonadotropin unit in the ovine fetus. Endocrinology 129:3073–3079PubMedGoogle Scholar
  21. Naess O, Haug E, Attramadal A, Aakvaag A, Hansson V, French F (1976) Androgen receptors in the anterior pituitary and central nervous system of the androgen “insensitive” (Tfm) rat: correlation between receptor binding and effects of androgens on gonadotropin secretion. Endocrinology 99:1295–1303PubMedGoogle Scholar
  22. Nishihara E, Nagayama Y, Inoue S, Hiroi H, Muramatsu M, Yamashita S, Koji T (2000) Ontogenetic changes in the expression of estrogen receptor alpha and beta in rat pituitary gland detected by immunohistochemistry. Endocrinology 141:615–620PubMedCrossRefGoogle Scholar
  23. Ochiai I, Matsuda KI, Nishi M, Ozawa H, Kawata M (2004) Imaging analysis of subcellular correlation of androgen receptor and estrogen receptor in single living cells using green fluorescent protein color variants. Mol Endocrinol 18:26–42PubMedCrossRefGoogle Scholar
  24. Okada Y, Fujii Y, Moore JP Jr, Winters SJ (2003) Androgen receptors in gonadotrophs in pituitary cultures from adult male monkeys and rats. Endocrinology 144:267–273PubMedCrossRefGoogle Scholar
  25. Panet-Raymond V, Gottlieb B, Beitel LK, Pinsky L, Trifiro MA (2000) Interactions between androgen and estrogen receptors and the effects on their transactivational properties. Mol Cell Endocrinol 167:139–150PubMedCrossRefGoogle Scholar
  26. Schanbacher BD, Johnson MP, Tindall DJ (1987) Androgenic regulation of luteinizing hormone secretion: relationship to androgen binding in sheep pituitary. Biol Reprod 36:340–350PubMedCrossRefGoogle Scholar
  27. Scully KM, Gleiberman AS, Lindzey J, Lubahn DB, Korach KS, Rosenfeld MG (1997) Role of estrogen Receptor-alpha in the Anterior Pituitary Gland. Mol Endocrinol 11:674–681PubMedCrossRefGoogle Scholar
  28. Sheng C, McNeilly AS, Brookst AN (1998) Immunohistochemical distribution of oestrogen receptor and luteinizing hormone β subunit in the ovine pituitary gland during fetal development. J neuroendocrinol 10:713–718PubMedCrossRefGoogle Scholar
  29. Stefaneanu L (1997) Pituitary sex steroid receptors: localization and function. Endocr Pathol 8:91–108PubMedGoogle Scholar
  30. Tian X, Cui S, Liu J, Yi S (2004) Expression of estrogen receptors in the efferent ductile of male sheep fetuses during gestation. Histochem Cell Biol 122:473–475PubMedCrossRefGoogle Scholar
  31. Tilbrook AJ, Clarke IJ (2001) Negative feedback regulation of the secretion and actions of gonadotropin-releasing hormone in males. Biol Reprod 64:735–742PubMedCrossRefGoogle Scholar
  32. Wersinger SR, Haisenleder DJ, Lubahn DB, Rissman EF (1999) Steroid feedback on gonadotropin release and pituitary gonadotropin subunit mRNA in mice lacking a functional estrogen receptor alpha. Endocrine 11:137–143PubMedCrossRefGoogle Scholar
  33. Woods JE, Blandin CM, Thommes RC (1994) Ontogeny of immunocytochemically demonstrable androgen and androgen receptor-containing cells in the hypothalamus and adenohypophysis of the chick embryo. Growth Dev Aging 58:21–31PubMedGoogle Scholar
  34. Woods JE, Otten LM, Thommes RC (1995) Ontogeny of 17B-estradiol (E2) and estrogen receptor (ER)-immunostained cells in the hypothalamus and adenohyophyseal pars distalis of the chick embryo. Growth Dev Aging 59:93–105PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • XueJun Yuan
    • 1
    • 2
  • YuQin He
    • 3
  • JiaLi Liu
    • 1
  • HaoShu Luo
    • 1
  • JinHua Zhang
    • 1
  • Sheng Cui
    • 1
  1. 1.Department of Animal Physiology, College of Biological SciencesChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.College of Biological SciencesShandong Agricultural UniversityTaianPeople’s Republic of China
  3. 3.Gansu Agricultural UniversityLanzhouPeople’s Republic of China

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