Expression of androgen receptor and its co-localization with estrogen receptor-alpha in the developing pituitary gland of sheep fetus
- 148 Downloads
No information is known concerning the expression of androgen receptor (AR) and its co-localization with estrogen receptor alpha (ERα) in the developing pituitary of sheep fetus. In the present study, we detected AR expression and its co-localization with ERα in the anterior pituitary of sheep fetus from day 60 of gestation to the postnatal by dual immunochemistry. The results showed that both AR immunoreactivity (AR-ir) and ERα immunoreactivity (ERα-ir) were predominantly localized in the nuclei of LH positive gonadotropes. The cell counting results showed that the percentage of the anterior pituitary cells expressing AR fluctuated from 13.51 ± 0.92 to 17.05 ± 1.83% during the examined stages, but there were no significant differences between sexes and among ages examined (P > 0.05). However, the proportion of AR-ir cells containing LH markedly increased from day 60 of gestation to the neonatal (P < 0.05). The percentage of AR-ir cells expressing ERα-ir significantly increased from day 60 of gestation to the neonatal, respectively (P < 0.05), but no significant differences were seen between genders at each stage examined. These results indicate that both AR and ERα are mainly expressed in the gonadotropes of anterior pituitary gland of sheep fetuses, whereas the functions and interaction of AR and ERα expressions in the developing pituitary gland are required to be elucidated further.
KeywordsImmunohistochemistry AR ERα Sheep fetus Pituitary
This work was supported from grants of the Natural Science Foundation of Beijing (6042013) and the Natural Science Foundation of China (30325034, 30471264). We thank Prof. AS McNeilly (Centre for reproductive biology, Edinburgh, UK) for the kind gift of rabbit anti-ovine LH and PRL antibody. We also thank Professor GL Xia, Department of Animal Physiology, College of Biological Sciences, China Agricultural University, People’s Republic of China for reviewing the English manuscript. Anti-ovine GH, anti-ovine TSH and anti-human ACTH primary antibodies were obtained through the National Hormone and Peptide Program (NHPP), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) & Dr Parlow (NHPP).
- Lindzey J, Wetsel WC, Couse JF, Stoker T, Cooper R, Korach KS (1998) Effects of Castration and chronic steroid treatments on hypothalamic gonadotropin-releasing hormone content and pituitary gonadotropins in male wild-type and estrogen receptor-α knockout mice. Endocrinology 139:4092–4101PubMedCrossRefGoogle Scholar
- Naess O, Haug E, Attramadal A, Aakvaag A, Hansson V, French F (1976) Androgen receptors in the anterior pituitary and central nervous system of the androgen “insensitive” (Tfm) rat: correlation between receptor binding and effects of androgens on gonadotropin secretion. Endocrinology 99:1295–1303PubMedGoogle Scholar