Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates
- 151 Downloads
Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.
KeywordsNuclear myosin I Myosin 1c Transcription Chromatin Tissue expression
We are grateful to Peter G. Gillespie for the anti-Myo1c antibody, Lenka Rossmeislová and Zdeněk Hodný for critically reading the manuscript. This work was supported by the Grant Agency of Czech Republic (reg. no. 204/04/0108), Grant Agency of the Academy of Sciences of the Czech Republic (reg. No. IAA5039202), grant LC545 of the MSMT, and by the institutional grant no. AV0Z50390512. MŠ and RD were supported by the student program of the Grant Agency of the Czech Republic (reg. No. 204/05/H023 IV).
- Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, Bouneau L, Fischer C, Ozouf-Costaz C, Bernot A, Nicaud S, Jaffe D, Fisher S, Lutfalla G, Dossat C, Segurens B, Dasilva C, Salanoubat M, Levy M, Boudet N, Castellano S, Anthouard V, Jubin C, Castelli V, Katinka M, Vacherie B, Biemont C, Skalli Z, Cattolico L, Poulain J, De Berardinis V, Cruaud C, Duprat S, Brottier P, Coutanceau JP, Gouzy J, Parra G, Lardier G, Chapple C, McKernan KJ, McEwan P, Bosak S, Kellis M, Volff JN, Guigo R, Zody MC, Mesirov J, Lindblad-Toh K, Birren B, Nusbaum C, Kahn D, Robinson-Rechavi M, Laudet V, Schachter V, Quetier F, Saurin W, Scarpelli C, Wincker P, Lander ES, Weissenbach J, Roest Crollius H (2004) Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype. Nature 431:946–957PubMedCrossRefGoogle Scholar
- Yanai I, Benjamin H, Shmoish M, Chalifa-Caspi V, Shklar M, Ophir R, Bar-Even A, Horn-Saban S, Safran M, Domany E, Lancet D, Shmueli O (2005) Genome-wide midrange transcription profiles reveal expression level relationships in human tissue specification. Bioinformatics 21:650–659PubMedCrossRefGoogle Scholar
- Yildirim A, Whish WJ (1997) The role of protein secretion on the adhesion strength of Chinese hamster lung (CHL) cells. Biochem Soc Trans 25:397–405Google Scholar