Histochemistry and Cell Biology

, Volume 123, Issue 4–5, pp 441–446

Human sterol 12α-hydroxylase (CYP8B1) is mainly expressed in hepatocytes in a homogenous pattern

  • Jin Wang
  • Sinead Greene
  • Lennart C. Eriksson
  • Björn Rozell
  • Eva Reihnér
  • Curt Einarsson
  • Gösta Eggertsen
  • Mats Gåfvels
Original Paper

DOI: 10.1007/s00418-005-0779-0

Cite this article as:
Wang, J., Greene, S., Eriksson, L.C. et al. Histochem Cell Biol (2005) 123: 441. doi:10.1007/s00418-005-0779-0

Abstract

The liver is the only organ where the complete synthesis of bile acids takes place. The present study was undertaken to investigate whether regional differences exist within the individual human hepatic lobuli regarding the pattern of expression of sterol 12α-hydroxylase (CYP8B1), a key enzyme in bile acid synthesis. A specific anti-human CYP8B1 peptide antiserum was developed and used for Western blotting and hepatic immunostaining of livers from various patients. CYP8B1 in human liver was expressed in the cytoplasm of hepatocytes with an even nonzonal distribution within the liver lobulus. Pericentral expression was confirmed for CYP2E1. A weak staining was noted in cholangiocytes and Kupffer cells. Previous studies on hepatic CYP27A1 and CYP7A1 in rats have shown a zonal expression, primarily in the pericentral region. Our studies indicate a different pattern for CYP8B1 expression in human liver, which was even rather than zonal.

Keywords

CYP8B1 Bile acids Immunohistochemistry Liver 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Jin Wang
    • 1
    • 4
  • Sinead Greene
    • 1
  • Lennart C. Eriksson
    • 3
  • Björn Rozell
    • 2
    • 3
  • Eva Reihnér
    • 4
  • Curt Einarsson
    • 4
  • Gösta Eggertsen
    • 1
  • Mats Gåfvels
    • 1
  1. 1.Department of Laboratory Medicine, Division of Clinical ChemistryKarolinska University Hospital HuddingeStockholmSweden
  2. 2.Division of Clinical Research CenterKarolinska University Hospital HuddingeStockholmSweden
  3. 3.Division of PathologyKarolinska University Hospital HuddingeStockholmSweden
  4. 4.Department of MedicineKarolinska University Hospital HuddingeStockholmSweden

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