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Histochemistry and Cell Biology

, Volume 125, Issue 3, pp 315–324 | Cite as

Tissue distribution of the secretory protein, SPLUNC1, in the human fetus

  • Hou-De Zhou
  • Song-Qin Fan
  • Jin Zhao
  • Dong-Hai Huang
  • Ming Zhou
  • Hua-Ying Liu
  • Zhao-Yang Zeng
  • Yi-Xing Yang
  • He Huang
  • Xiao-Ling Li
  • Shou-Rong Shen
  • Gui-Yuan Li
Original paper

Abstract

We previously identified a tissue-specific gene, short palate, lung, and nasal epithelium clone 1 (SPLUNC1), in nasopharyngeal epithelial tissues. SPLUNC1 was differentially expressed in nasopharyngeal carcinoma. Bioinformatic analysis revealed that SPLUNC1 has the bactericidal permeability-increasing protein/lipid-binding protein (BPI/LBP) domain and a 19 amino acid signal peptide, which suggest that it is a secretory protein. Its precise cellular localization in the respiratory tract is mainly in mucous cells and ducts of submucosal glands. However, little is known about its expression pattern in various human tissues. We generated a highly specific antibody and analyzed its distribution in the human fetus by immunohistochemistry to more precisely determine SPLUNC1 protein localization in human tissues. The results were further validated by RT-PCR. Our results showed that SPLUNC1 protein is expressed at not only the serous glands and epithelium of the upper respiratory tract and digestive tract, but also in the oculi of human embryos. Interestingly, we also found positive staining in fetus adipose tissue, a result not previously reported in studies of adult human tissues. Western blot analysis detected a 24 kDa SPLUNC1 protein in the compounds of nasopharyngeal secretions. This secretory protein was also detected in saliva and tears. Our research suggests that SPLUNC1 protein may not only be an antimicrobial peptide that plays an important role in the maintenance of homeostasis in the upper respiratory tract, oculi, and alimentary tract, it may also be important in the development and lipid metabolism of the adipose tissue.

Keywords

SPLUNC1 Expression pattern Secretory protein Epithelial 

Notes

Acknowledgements

This work was supported partially by National Natural Science Foundation (30200312, 30300205).

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Hou-De Zhou
    • 1
  • Song-Qin Fan
    • 1
  • Jin Zhao
    • 1
  • Dong-Hai Huang
    • 2
  • Ming Zhou
    • 1
  • Hua-Ying Liu
    • 1
  • Zhao-Yang Zeng
    • 1
  • Yi-Xing Yang
    • 1
  • He Huang
    • 1
  • Xiao-Ling Li
    • 1
  • Shou-Rong Shen
    • 3
  • Gui-Yuan Li
    • 1
  1. 1.Cancer Research InstituteCentral South UniversityChangsha, HunanChina
  2. 2.XiangYa HospitalCentral South UniversityChangsha, HunanChina
  3. 3.the Third XiangYa HospitalCentral South UniversityChangsha, HunanChina

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