Histochemistry and Cell Biology

, Volume 125, Issue 1–2, pp 91–102 | Cite as

Replication of centromeric heterochromatin in mouse fibroblasts takes place in early, middle, and late S phase

  • Stefanie Weidtkamp-Peters
  • Hans-Peter Rahn
  • M. Cristina Cardoso
  • Peter Hemmerich
Original paper

Abstract

The replication of eukaryotic chromosomes takes place throughout S phase, but little is known how this process is organized in space and time. Early and late replicating chromosomal domains appear to localize to distinct spatial compartments of the nucleus where DNA synthesis can take place at defined times during S phase. In general, transcriptionally active chromatin replicates early in S phase whereas transcriptionally inactive chromatin replicates later. Here we provide evidence for significant deviation from this dogma in mouse NIH3T3 cells. While the bulk pericentromeric heterochromatin replicates exclusively during mid to late S phase, centromeric DNA domains associated with constitutive kinetochore proteins are replicated throughout all stages of S phase. On an average, 12±4% of centromeres replicate in early S phase. Early replication of a subset of centromeres was also detected in living C2C12 murine cells. Thus, in contrast to expectation, late replication is not an obligatory feature of centromeric heterochromatin in murine cells and it does not determine their ‘heterochromatic state’.

Keywords

Heterochromatin Centromers Replication Cell cycle Chromocenters 

Supplementary material

418_2005_63_MOESM1_ESM.mov (1.7 mb)
Supplementary materials

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Stefanie Weidtkamp-Peters
    • 1
  • Hans-Peter Rahn
    • 2
  • M. Cristina Cardoso
    • 2
  • Peter Hemmerich
    • 1
  1. 1.Institute of Molecular BiotechnologyJenaGermany
  2. 2.Max Delbrück CentrumBerlinGermany

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