Histochemistry and Cell Biology

, Volume 121, Issue 3, pp 201–207 | Cite as

Localization of the Na+-d-glucose cotransporter SGLT1 in the blood-brain barrier

  • Katrin Elfeber
  • Alwin Köhler
  • Michael Lutzenburg
  • Christina Osswald
  • Hans-Joachim Galla
  • Otto W. Witte
  • Hermann Koepsell
Original Paper

Abstract

Immunoreactivity of the Na+-d-glucose cotransporter SGLT1 was demonstrated in intracerebral capillaries of rat and pig. Immunostaining suggested that SGLT1 is located in the luminal membrane of the endothelial cells and in intracellular vesicles. Using in situ hybridization, SGLT1 mRNA was not detectable in intracerebral capillaries of non-treated or sham-operated Wistar rats. However, 1 day after a transient occlusion of the right middle cerebral artery, SGLT1 mRNA was detected in capillaries of both brain hemispheres. Expression of SGLT1 was also demonstrated in primary cultures of capillary endothelial cells from pig using polymerase chain reaction after reverse transcription and western blotting. The data suggest that SGLT1 participates in transport of d-glucose across the blood-brain barrier and is upregulated after brain ischemia and reperfusion.

Keywords

Sugar transport Sodium d-glucose cotransporter SGLT1 Blood-brain barrier Brain ischemia 

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Katrin Elfeber
    • 1
  • Alwin Köhler
    • 1
  • Michael Lutzenburg
    • 2
  • Christina Osswald
    • 1
  • Hans-Joachim Galla
    • 3
  • Otto W. Witte
    • 2
  • Hermann Koepsell
    • 1
  1. 1.Institute of Anatomy and Cell BiologyUniversity WürzburgWürzburgGermany
  2. 2.Clinic of NeurologyUniversity DüsseldorfDüsseldorfGermany
  3. 3.Institute of BiochemistryMünsterGermany

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