Histochemistry and Cell Biology

, Volume 120, Issue 4, pp 285–292 | Cite as

Temporal and spatial assembly of lipid droplet-associated proteins in 3T3-L1 preadipocytes

  • Seu-Mei WangEmail author
  • Ran-Der Hwang
  • Andrew S. Greenberg
  • Hui-Ling Yeo
Original Paper


This study aimed to investigate the relationship between newly formed lipid droplets and lipid droplet surface proteins, including perilipin, adipose differentiation related protein (ADRP), and p200 kDa protein (p200) in 3T3-L1 preadipocytes, during lipogenesis. Sterol ester was used to induce nascent lipid droplets in 3T3-L1 preadipocytes and the sequence of lipids and lipid droplet surface proteins was studied using a combination of immunohistochemistry and Nile red staining/Oil red O. We demonstrated that, although most growing lipid droplets appeared to have a lipid core surrounded by a fluorescent rim of ADRP, perilipin, and p200, tiny protein aggregates of ADRP, perilipin, or p200 could also be found to occur in the absence of lipid accumulation. In addition, ADRP associated with nascent lipid droplets prior to that of perilipin or p200. We provide evidence that lipid droplet surface proteins, especially ADRP and perilipin, are important in serving as a nucleation center for the assembly of lipid to form nascent lipid droplets.


ADRP Perilipin p200 Lipogenesis 



We thank Dr. Thomas Keenan for providing the rabbit anti-ADRP antibody, and Dr. Thomas Barkas for his critical reading and correction of this paper. The grant sponsor was the National Science Council, Republic of China, grant number NSC 91-2320-B-002-125


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Seu-Mei Wang
    • 1
    Email author
  • Ran-Der Hwang
    • 1
  • Andrew S. Greenberg
    • 2
  • Hui-Ling Yeo
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of MedicineNational Taiwan UniversityTaipeiTaiwan
  2. 2.United States Department of AgricultureHuman Nutrition Research Center on Aging at Tufts UniversityBostonUSA

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