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Histochemistry and Cell Biology

, Volume 120, Issue 2, pp 111–119 | Cite as

Dissociation of mammalian Polycomb-group proteins, Ring1B and Rae28/Ph1, from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase

  • Hiro Miyagishima
  • Kyoichi Isono
  • Yuichi Fujimura
  • Masaomi Iyo
  • Yoshihiro Takihara
  • Hiroshi Masumoto
  • Miguel Vidal
  • Haruhiko Koseki
Original Paper

Abstract

The Polycomb group (PcG) gene products form complexes that regulate chromatin configuration to mediate cellular memory to postmitotic somatic cells and postmeiotic oocytes in Drosophila melanogaster. Structural and functional similarities of PcG proteins between invertebrates and vertebrates suggest mammalian PcG proteins may be involved to imprint transcriptional status at various loci into postmitotic and postmeiotic daughter cells. To address molecular mechanisms underlying PcG-mediated cellular memory, it might be a prerequisite to understand subcellular localization of PcG proteins during mitosis and meiosis. In this study, we analyzed subcellular localization of Rae28/Ph1 and Ring1B by using newly generated monoclonal antibodies in mitotic somatic cells and meiotic mouse oocytes. Results suggest that Rae28/Ph1 and Ring1B dissociate from the chromatin upon its condensation in mitotic prophase in the U2-OS human osteosarcoma cell line. During maturation of oocytes, significant alterations of Rae28/Ph1 and Ring1B localization are concordant with configuration changes of the chromatin at the germinal vesicle stage of meiotic prophase. Importantly, dissociation of Rae28/Ph1 and Ring1B from the chromatin temporally correlates with transcriptional arrest both in mitosis and meiosis. Present and previous observations suggest molecular mechanisms required for mitotic regulation of RNA polymerase II could be involved in dissociation of PcG proteins.

Keywords

Polycomb Mitosis Oocyte Mammals Chromatin 

Notes

Acknowledgements

We are grateful to Drs. M. Maekawa and Y. Toyama for the valuable suggestions on histological analyses of ovaries, Ms Sanae Takeda and Misao Uchida and Mr. Shozo Sugimori for their technical assistance, and Dr. T. Akasaka for initial instructions for confocal microscopic analyses. This project was supported by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Hiro Miyagishima
    • 1
    • 2
  • Kyoichi Isono
    • 1
  • Yuichi Fujimura
    • 1
  • Masaomi Iyo
    • 2
  • Yoshihiro Takihara
    • 3
  • Hiroshi Masumoto
    • 4
  • Miguel Vidal
    • 5
  • Haruhiko Koseki
    • 1
    • 6
  1. 1.Department of Molecular Embryology, Graduate School of MedicineChiba UniversityChibaJapan
  2. 2.Department of Psychiatry, Graduate School of MedicineChiba UniversityChibaJapan
  3. 3.Department of Developmental Biology and MedicineOsaka Medical Center for Cancer and Cardiovascular DiseasesOsakaJapan
  4. 4.Division of Biological Science, Graduate School of ScienceNagoya UniversityNagoyaJapan
  5. 5.Centro de Investigaciones BiologicasDepartment of Development and Cell BiologyMadridSpain
  6. 6.RIKEN Research Center for Allergy and ImmunologyYokohamaJapan

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