Histochemistry and Cell Biology

, Volume 120, Issue 1, pp 51–61 | Cite as

Protein kinase C isoforms in the enteric nervous system

  • Daniel P. Poole
  • Billie Hunne
  • Heather L. Robbins
  • John B. Furness
Original Paper
First Online:
Accepted:

Abstract

C kinases (PKCs) are a family of enzymes essential for the transduction of signals in a diverse range of cell types, including neurons. The different isoforms vary in their activation requirements. Therefore, cell-specific expression of different isoforms has implications for PKC-mediated control of organ function. This study has investigated the types and distributions of PKC isoforms in the small intestine of the guinea-pig, with particular emphasis on their localisation in myenteric neurons, using immunohistochemistry and western blotting techniques. Three PKC isoforms, γ, η and θ, were detected in the calbindin-immunoreactive subset of intrinsic primary afferent neurons, but not in other myenteric neurons. Both γ and θ immunoreactivities were also located in interstitial cells of Cajal. In contrast to these isoforms, immunoreactivity for PKCs λ and ε was present in all myenteric neurons of the ileum. PKCα immunoreactivity was detected primarily in the glial network, as shown through double labelling with antibodies to the glial filament protein, S100b. Myenteric neurons were also weakly immunoreactive for this isoform. PKCδ immunoreactivity was very highly expressed in smooth muscle, but was largely absent from neurons. Immunoreactivity for RACK1, a binding protein for PKCβ, was detected in both calbindin-immunoreactive neurons and in smooth muscle cells. This study indicates a selective distribution of PKC isoforms to specific cell types. Isoform-specific activity of these enzymes could provide a means through which targeted modulation of intestinal function is achieved.

Keywords

Protein kinase C Enteric nervous system Sensory neuron Interstitial cells of Cajal Calbindin 
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Notes

Acknowledgements

This work was funded by the National Health and Medical Research Council of Australia.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Daniel P. Poole
    • 1
  • Billie Hunne
    • 1
  • Heather L. Robbins
    • 1
  • John B. Furness
    • 1
  1. 1.Department of Anatomy and Cell Biology and Centre for NeuroscienceUniversity of MelbourneParkville, VIC 3010Australia

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