Effect of topical povidone-iodine versus topical ofloxacin on experimental Staphylococcus keratitis

  • Samir A. Melki
  • Ammar Safar
  • Farzad Yaghouti
  • Philip Scharper
  • M. Scharper
  • Barbara Zeligs
  • Ana Luiza MacDowell
  • Michael A. Goldberg
  • Jay Lustbader
Laboratory Investigation

Abstract 

Purpose: To compare the antibacterial effect of povidone-iodine (PI) with that of ofloxacin in an experimental model of bacterial keratitis.Methods: Staphyloccocal keratitis was induced in 21 eyes of Dutch Belted rabbits by intrastromal inoculation of approximately 280 organisms of Staphylococcus aureus. Six hours later, the animals were divided in four groups treated topically with saline 0.9%, Betadine 10%, Betadine 0.5% or Ofloxacin 0.3% (2 gtt every 30 min for 8 h). The central 8-mm cornea was excised, washed and homogenized. Colony counts were performed on serial 10-fold dilutions plated on blood and brain infusion agar and incubated overnight.Results: Colony-forming units per cornea were 7.4×107 for the saline group compared to 8.2×107 for PI 10% (P>0.5), 4.3×107 for PI 0.5% (P<0.01) and no organisms for ofloxacin 0.3%.Conclusions: Betadine 0.5% demonstrates a statistically significant bactericidal effect compared with untreated staphyloccocal keratitis in our experimental model. Ofloxacin has superior antibacterial effect under the conditions studied. Further improvements in the povidone-iodine formulation are warranted prior to consideration for human keratitis.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Samir A. Melki
    • 1
  • Ammar Safar
    • 1
  • Farzad Yaghouti
    • 1
  • Philip Scharper
    • 1
  • M. Scharper
    • 1
  • Barbara Zeligs
    • 3
  • Ana Luiza MacDowell
    • 3
  • Michael A. Goldberg
    • 1
  • Jay Lustbader
    • 1
  1. 1.Department of Ophthalmology, Georgetown University Medical Center, Washington DC, USAUS
  2. 2.Cornea and Refractive Surgery Service, Massachusetts Eye and Ear Infirmary, Harvard Medical School. 243 Charles Street, Boston, MA 02114, USA e-mail: Melki@rcn.com Tel.: +1-617-5574686 Fax: +1-617-5734300US
  3. 3.Microbiology Department, Georgetown University Medical Center, Washington DC, USAUS

Personalised recommendations