Advertisement

Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis

  • Hironori Migita
  • Tomohiro Ueno
  • Tomoko Tsukahara-Kawamura
  • Yusuke Saeki
  • Nozomu Hanaoka
  • Tsuguto Fujimoto
  • Eiichi UchioEmail author
Inflammatory Disorders
  • 63 Downloads

Abstract

Background

We evaluated a novel silver amplification immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.

Methods

Two kinds of immunochromatographic (IC) kits, a conventional IC kit for conjunctival scrapings (control kit) and an IC kit using tears including conjunctival exudate collected by pressing a filter paper strip on the conjunctiva (test kit), were tested on 90 patients who attended Migita Eye Clinic with suspected adenoviral conjunctivitis. The results of the test kits were automatically obtained by a specific reader, which was based on silver amplification immunochromatography system, in 15 min. The detection of AdV was confirmed by real-time polymerase chain reaction (PCR) method, and typing was performed by direct sequencing. Comparative dilution assay was carried out with the two kits, using AdV type 3 and type 54 strains.

Results

The sensitivity of the control kit and test kit was 89.8% and 98.3%, respectively. The specificity of both kits was 100%. A significant difference in the sensitivities of the two IC kits against PCR positivity was observed (P < 0.01). A significant correlation was found between AdV DNA copy numbers on a logarithmic scale obtained with the two tests (P < 0.01). The sensitivity of the test kit was 32–64-fold higher than that of the control kit without silver amplification for both AdV types.

Conclusions

These results suggest that this novel amplified AdV detection kit using tears including conjunctival exudate is useful, because it decreases patients’ discomfort from specimen collection and its sensitivity is significantly higher than that of the conventional IC kit.

Keywords

Adenovirus Immunochromatography Silver amplification Tear 

Notes

Acknowledgements

The clinical strains of adenovirus type 3 and type 54 used for comparative dilution assay were kindly provided by the Kobe Institute of Health.

Funding information

This work was supported by a Grant-in-Aid for Encouragement of Scientists (18K09466) from the Ministry of Education, Science, Sports and Culture of Japan.

Compliance with ethical standards

Conflict of interest

Authors Migita, Tsukahara-Kawamura, Saeki, and Uchio were provided immunochromatography tests (Quick Chaser Auto Adeno Eye (Nitten Pharmaceutical Co., Ltd., Nagoya, Japan), FUJI DRI-CHEM IMMUNO AG cartridge Adeno OPH (FUJIFILM Corp., Tokyo, Japan)) and immuno reader (Quick Chaser® Immuno Reader (Mizuho Medy Co. Ltd., Tosu, Japan), FUJI DRI-CHEM IMMUNO AG1 (FUJIFILM Corp.)) from each company. Author Ueno declares that he has no conflict of interest. Author Hanaoka declares that he has no conflict of interest. Author Fujimoto declares that he has no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. 1.
    Zhang L, Zhao N, Sha J, Wang C, Jin X, Amer S, Liu S (2016) Virology and epidemiology analyses of global adenovirus-associated conjunctivitis outbreaks, 1953-2013. Epidemiol Infect 144:1661–1672CrossRefGoogle Scholar
  2. 2.
    Grayston JT, Johnston PB, Loosli CG, Smith ME (1956) An improved technique for the neutralization test with adenoviruses in HeLa cell cultures. J Infect Dis 99:188–198CrossRefGoogle Scholar
  3. 3.
    Miura-Ochiai R, Shimada Y, Konno T, Yamazaki S, Aoki K, Ohno S, Suzuki E, Ishiko H (2007) Quantitative detection and rapid identification of human adenoviruses. J Clin Microbiol 45:958–967CrossRefGoogle Scholar
  4. 4.
    Uchio E, Aoki K, Saitoh W, Itoh N, Ohno S (1997) Rapid diagnosis of adenoviral conjunctivitis on conjunctival swabs by 10-minute immunochromatography. Ophthalmology 104:1294–1299CrossRefGoogle Scholar
  5. 5.
    Ouchi K, Hasegawa K, Nonaka Y, Matsushima H, Komura H, Maki T, Nakazawa T (1999) Rapid diagnosis of adenovirus respiratory tract infections by immunochromatography. J Infect Chemother 5:220–222CrossRefGoogle Scholar
  6. 6.
    Wada A, Sakoda Y, Oyamada T, Kida H (2011) Development of a highly sensitive immunochromatographic detection kit for H5 influenza virus hemagglutinin using silver amplification. J Virol Methods 178:82–86CrossRefGoogle Scholar
  7. 7.
    Cho IH, Seo SM, Paek EH, Paek SH (2010) Immunogold-silver staining-on-a-chip biosensor based on cross-flow chromatography. J Chromatogr B Analyt Technol Biomed Life Sci 878:271–277CrossRefGoogle Scholar
  8. 8.
    Watanabe M, Kohdera U, Kino M, Haruta T, Nukuzuma S, Suga T, Akiyoshi K, Ito M, Suga S, Komada Y (2005) Detection of adenovirus DNA in clinical samples by SYBR Green real-time polymerase chain reaction assay. Pediatr Int 47:286–291CrossRefGoogle Scholar
  9. 9.
    Gu Z, Belzer SW, Gibson CS, Bankowski MJ, Hayden RT (2003) Multiplexed, real-time PCR for quantitative detection of human adenovirus. J Clin Microbiol 41:4636–4641CrossRefGoogle Scholar
  10. 10.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefGoogle Scholar
  11. 11.
    Vastine DW, Schwartz HS, Yamashiroya HM, Smith RF, Guth SB (1977) Cytologic diagnosis of adenoviral epidemic keratoconjunctivitis by direct immunofluorescence. Invest Ophthalmol Vis Sci 16:195–200Google Scholar
  12. 12.
    Schwartz HS, Vastine DW, Yamashiroya H, West CE (1976) Immunofluorescent detection of adenovirus antigen in epidemic keratoconjunctivitis. Investig Ophthalmol 15:199–207Google Scholar
  13. 13.
    Kobayashi TK, Sato S, Tsubota K, Takamura E (1991) Cytological evaluation of adenoviral follicular conjunctivitis by cytobrush. Ophthalmologica 202:156–160CrossRefGoogle Scholar
  14. 14.
    Versura P, Bonvicini F, Caramazza R, Laschi R (1985) Scanning electron microscopy study of human cornea and conjunctiva in normal and various pathological conditions. Scan Electron Microsc (Pt 4):1695–1708Google Scholar
  15. 15.
    Darougar S, Grey RH, Thaker U, McSwiggan DA (1983) Clinical and epidemiological features of adenovirus keratoconjunctivitis in London. Br J Ophthalmol 67:1–7CrossRefGoogle Scholar
  16. 16.
    Hoyle E, Erez JC, Kirk-Granger HR, Collins E, Tang JW (2016) An adenovirus 4 outbreak amongst staff in a pediatric ward manifesting as keratoconjunctivitis - a possible failure of contact and aerosol infection control. Am J Infect Control 44:602–604CrossRefGoogle Scholar
  17. 17.
    Boerner CF, Lee FK, Wickliffe CL, Nahmias AJ, Cavanagh HD, Straus SE (1981) Electron microscopy for the diagnosis of ocular viral infections. Ophthalmology 88:1377–1381CrossRefGoogle Scholar
  18. 18.
    Kaye SB, Lloyd M, Williams H, Yuen C, Scott JA, O’Donnell N, Batterbury M, Hiscott P, Hart CA (2005) Evidence for persistence of adenovirus in the tear film a decade following conjunctivitis. J Med Virol 77:227–2231CrossRefGoogle Scholar
  19. 19.
    Horton JC, Magnetic MS (2015) Resonance imaging in epidemic adenoviral keratoconjunctivitis. JAMA Ophthalmol 133:960–961CrossRefGoogle Scholar
  20. 20.
    Mimura T, Usui T, Mori M, Aixinjueluo W, Funatsu H, Noma H, Amano S (2010) Immunochromatographic assay for measurement of total IgE in tears, nasal mucus, and saliva of patients with allergic rhinoconjunctivitis. J Asthma 47:1153–1160CrossRefGoogle Scholar
  21. 21.
    Morozumi M, Shimizu H, Matsushima Y, Mitamura K, Tajima T, Iwata S, Ubukata K (2014) Evaluation of new immunochromatographic assay kit for adenovirus detection in throat swab: comparison with culture and real-time PCR results. J Infect Chemother 20:303–306CrossRefGoogle Scholar
  22. 22.
    Uemura T, Migita H, Ueno T, Tsukahara-Kawamura T, Saeki Y, Fujimoto T, Uchio E (2018) Clinical and virological analysis of epidemic keratoconjunctivitis caused by adenovirus type 54 in a regional ophthalmic clinic in Kyushu, Japan. Clin Ophthalmol 12:511–517CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Hironori Migita
    • 1
  • Tomohiro Ueno
    • 2
  • Tomoko Tsukahara-Kawamura
    • 2
  • Yusuke Saeki
    • 2
  • Nozomu Hanaoka
    • 3
  • Tsuguto Fujimoto
    • 3
  • Eiichi Uchio
    • 2
    Email author
  1. 1.Migita Eye ClinicChikushinoJapan
  2. 2.Department of OphthalmologyFukuoka University School of MedicineFukuokaJapan
  3. 3.Infectious Disease Surveillance CenterNational Institute of Infectious DiseasesTokyoJapan

Personalised recommendations