Topical application of autologous adipose-derived mesenchymal stem cells (MSCs) for persistent sterile corneal epithelial defect

  • George I. Agorogiannis
  • Vassilia-Ismini Alexaki
  • Ourania Castana
  • George D. Kymionis
Case Report


Different causes of corneal persistent epithelial defects (PED) have been described including dry eye, corneal epithelial stem cell deficiency, diabetes mellitus, and neurotrophic keratopathy (after herpetic keratitis or corneal surgery). Corneal PEDs could lead to a cascade of events throughout the cornea, resulting in stromal degradation, thinning and in advanced cases, corneal perforation.

Several treatment options have been proposed for corneal PEDs (based on the etiology) such as eye patching, unpreserved artificial tears, bandage contact lens, punctual plugging, tarsorrhaphy, autologous serum eyedrops, limbal stem cells transplantation, and amniotic membrane grafting.

Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types. Clinical uses of these cells have been proposed, such as in patients with degenerative joint disease (using percutaneously implanted autologous MSCs) [1], while adipose-derived MSCs have been...


Central Corneal Thickness Keratoconus Corrected Distance Visual Acuity Corneal Perforation Corneal Epithelial Defect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank the heads of the Ophthalmology and Experimental Endocrinology departments (University of Crete, School of Medicine, Heraklion, Greece), Professors Ioannis G Pallikaris and Elias Castanas, for their constant support and fruitful discussions, which stimulated the current study.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • George I. Agorogiannis
    • 1
  • Vassilia-Ismini Alexaki
    • 2
  • Ourania Castana
    • 3
  • George D. Kymionis
    • 1
    • 4
  1. 1.Eye ClinicUniversity Hospital of Heraklion, CreteHeraklionGreece
  2. 2.Laboratory of Experimental EndocrinologyUniversity of Crete, School of MedicineHeraklionGreece
  3. 3.Department of Plastic and Reconstructive SurgeryEvangelismos General HospitalAthensGreece
  4. 4.University of Crete, School of Medicine, Department of OphthalmologyUniversity Hospital of CreteHeraklionGreece

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