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Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy

  • Yvette Ducournau
  • Claude BoscherEmail author
  • Ron A. Adelman
  • Colette Guillaubey
  • Didier Schmidt-Morand
  • Jean-François Mosnier
  • Didier Ducournau
Miscellaneous

Abstract

Background

There is some in vitro evidence that the adult ciliary body might harbor an inactive population of stem/retinal progenitor cells (RPC), or that ciliary epithelial (CE) cells might have the capacity to trans-differentiate, which may result in a balance between neural and epithelial properties. We have reported alterations in the ciliary body (CB) and adjacent vitreous in vivo by endoscopic evaluation of human eyes with a history of retinal detachment (RD) and anterior proliferative vitreoretinopathy (PVR).

Methods

The present study examined with light microscopy three paraffin–embedded phthisic human eyes with RD and anterior PVR. One normal eye, exenterated for an orbital tumor, served as the control. All specimens were stained with hematoxilin and eosin safran (HES), and serial sections were immunostained with antibodies against EGFR, Ki67, CD133, NSE, rhodopsin, and GFAP.

Results

We observed: (1) an intense proliferation and displacement of clusters of CE cells into the vitreous base in a “neurosphere-like” fashion; (2) differentiation of CE cells towards early and late neuronal [photoreceptor (PR)] lineages; and (3) strong staining of EGF and EGFR in the CE. Such proliferation, migration, and differentiation were not present in the CE of the control eye. GFAP staining was intensely positive in the three detached retinae, and was negative in the CE of eyes with RD, as well as in the retina of the control eye.

Conclusions

Our observations suggest that EGFR-positive CE cells in the adult human eye in vivo with RD and PVR form “neurosphere-like” structures; their differentiation seems to be directed towards the neural and photoreceptor lineage, and not towards glial formation. In the adult human eye, the CE in a pathological retinal environment such as RD might provide a spontaneous source of donor cells for retinal transplantation.

Keywords

Ciliary margin Retinal detachment Proliferative vitreoretinopathy Endoscopic vitreoretinal surgery Retinal progenitor cells Retinal transplantation 

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yvette Ducournau
    • 1
  • Claude Boscher
    • 2
    Email author
  • Ron A. Adelman
    • 3
    • 8
  • Colette Guillaubey
    • 4
  • Didier Schmidt-Morand
    • 5
  • Jean-François Mosnier
    • 1
    • 7
  • Didier Ducournau
    • 6
  1. 1.Service d’Anatomie et Cytologie PathologiquesHôpital G et R LaennecCHU de NantesFrance
  2. 2.American Hospital of ParisNeuilly sur SeineFrance
  3. 3.Department of Ophthalmology and Visual ScienceYale University School of MedicineNew HavenUSA
  4. 4.Institut d’Anatomie Pathologie du ForezSaint-Etienne cedex 1France
  5. 5.Ecole Vétérinaire de Nantes, Atlanpôle - La ChantrerieNantesFrance
  6. 6.Clinique SourdilleNantes cedex 3France
  7. 7.Service d’Anatomie Pathologique BCentre Hospitalo-Universitaire de Nantes, Hopital G & R LaennecNantes cedex 1France
  8. 8.Yale University Eye CenterNew HavenUSA

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