Longterm cultures of the aged human RPE do not maintain epithelial morphology and high transepithelial resistance

  • Boris V. Stanzel
  • Mark S. Blumenkranz
  • Susanne Binder
  • Michael F. Marmor
Letter to the Editor

Keywords

ARPE19 Cell Corneal Endothelial Cell Transepithelial Resistance Bovine Corneal Endothelial Cell Culture Condition Variant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by an E. Schrödinger Fellowship grant (J2463- B13) from the Austrian Science Foundation to BVS, and with A. Rabensteiner Awards in 2005 and 2006 to BVS from the Austrian Ophthalmic Society. The authors wish to thank Jane Hu and Dr. Dean Bok, JSEI/UCLA for advice on culture media formulation and fetal RPE cell culture techniques. Miri Englander assisted with RPE culture maintenance. Dr. Itaya from the Zarbin laboratory is gratefully acknowledged for providing the silicone scraping spatula.

References

  1. 1.
    Binder S, Stanzel BV, Krebs I, Glittenberg C (2007) Transplantation of the RPE in AMD. Prog Retin Eye Res 26:516–554PubMedCrossRefGoogle Scholar
  2. 2.
    Hu J, Bok D (2001) A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Mol Vis 7:14–19PubMedGoogle Scholar
  3. 3.
    Luo Y, Zhuo Y, Fukuhara M, Rizzolo LJ (2006) Effects of culture conditions on heterogeneity and the apical junctional complex of the ARPE-19 cell line. Invest Ophthalmol Vis Sci 47:3644–3655PubMedCrossRefGoogle Scholar
  4. 4.
    Campochiaro PA, Hackett SF (1993) Corneal endothelial cell matrix promotes expression of differentiated features of retinal pigmented epithelial cells: implication of laminin and basic fibroblast growth factor as active components. Exp Eye Res 57:539–547PubMedCrossRefGoogle Scholar
  5. 5.
    McKay BS, Burke JM (1994) Separation of phenotypically distinct subpopulations of cultured human retinal pigment epithelial cells. Exp Cell Res 213:85–92PubMedCrossRefGoogle Scholar
  6. 6.
    Pfeffer BA (1991) Improved methodology for cell culture of human and monkey retinal pigment epithelium. Prog Retin Res 10:251–291CrossRefGoogle Scholar
  7. 7.
    Peng S, Rahner C, Rizzolo LJ (2003) Apical and basal regulation of the permeability of the retinal pigment epithelium. Invest Ophthalmol Vis Sci 44:808–817PubMedCrossRefGoogle Scholar
  8. 8.
    Chang CW, Ye L, Defoe DM, Caldwell RB (1997) Serum inhibits tight junction formation in cultured pigment epithelial cells. Invest Ophthalmol Vis Sci 38:1082–1093PubMedGoogle Scholar
  9. 9.
    von Recum HA, Okano T, Kim SW, Bernstein PS (1999) Maintenance of retinoid metabolism in human retinal pigment epithelium cell culture. Exp Eye Res 69:97–107CrossRefGoogle Scholar
  10. 10.
    Villarroel M, Garcia-Ramirez M, Corraliza L, Hernandez C, Simo R (2009) Effects of high glucose concentration on the barrier function and the expression of tight junction proteins in human retinal pigment epithelial cells. Exp Eye Res 89:913–920PubMedCrossRefGoogle Scholar
  11. 11.
    Rizzolo LJ, Chen X, Weitzman M, Sun R, Zhang H (2007) Analysis of the RPE transcriptome reveals dynamic changes during the development of the outer blood-retinal barrier. Mol Vis 13:1259–1273PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Boris V. Stanzel
    • 1
    • 2
    • 4
  • Mark S. Blumenkranz
    • 1
  • Susanne Binder
    • 2
    • 3
  • Michael F. Marmor
    • 1
  1. 1.Department of OphthalmologyStanford University School of MedicineStanfordUSA
  2. 2.The Ludwig Boltzmann Institute for Retinology and Biomicroscopic Laser SurgeryViennaAustria
  3. 3.Department of OphthalmologyRudolph Foundation ClinicViennaAustria
  4. 4.University Eye Hospital BonnBonnGermany

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