A prospective study determining the efficacy of topical 0.5% levofloxacin on bacterial flora of patients with chronic blepharoconjunctivitis

  • Yazmin Yactayo-Miranda
  • Christopher N. Ta
  • Lisa He
  • Thomas C. Kreutzer
  • Martin M. Nentwich
  • Anselm Kampik
  • Herminia Mino de Kaspar



To determine the efficacy of conjunctival bacterial eradication following a 1-day, 3-day and 7-day application of topical 0.5% levofloxacin, with and without eyelid scrub, in patients with chronic blepharoconjunctivitis (CBC).


Patients with CBC (n = 60) were prospectively randomized to three groups (n = 20 in each group): no antibiotic treatment, topical levofloxacin four times per day in both eyes, and eyelid scrub in addition to topical levovofloxacin four times a day in both eyes. Patients without CBC (n = 40) were enrolled as the negative control group. Cultures of the conjunctiva were obtained from both eyes at baseline, 1 day, 3 days, and 7 days following treatment.


The most common bacteria isolated for all groups at baseline were coagulase-negative Staphylococcus. Eight patients did not complete the study. The remaining 52 patients with CBC had a significantly higher rate of positive thioglycolate broth cultures (94%) compared to a 58% positive culture rate in patient without CBC (P < 0.0001). Treatment with at least 3 days of topical antibiotic in patient with CBC resulted in a significant reduction (P < 0.05) in the number of thioglycolate positive cultures (≤ 60%) compared to non-treated eyes (≥ 88%). Following a minimum of a 1-day application of antibiotic, the median colony-forming unit was 0–1 compared to 3–8 for eyes without antibiotic treatment (P < 0.05). Scrubbing of the eyelids did not provide further benefit compared to antibiotic treatment alone.


CBC eyes have a significantly higher number of positive cultures than eyes without CBC. The application of topical 0.5% levofloxacin for at least 3 days provided a significant reduction in the number of positive cultures as well as the number of bacteria harbored on the conjunctival surface.


Blepharitis Blepharoconjunctivitis Levofloxacin Fluoroquinolones Conjunctival flora 


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yazmin Yactayo-Miranda
    • 1
  • Christopher N. Ta
    • 2
  • Lisa He
    • 2
  • Thomas C. Kreutzer
    • 1
  • Martin M. Nentwich
    • 1
  • Anselm Kampik
    • 1
  • Herminia Mino de Kaspar
    • 1
    • 2
  1. 1.Department of OphthalmologyLudwig-Maximilians UniversityMunichGermany
  2. 2.Department of Ophthalmology, School of MedicineStanford UniversityStanfordUSA

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