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Hyalocyte proliferation and ECM accumulation modulated by bFGF and TGF-β1

  • Florian Sommer
  • Klaus Pollinger
  • Ferdinand Brandl
  • Barbara Weiser
  • Jörg Teßmar
  • Torsten Blunk
  • Achim Göpferich
Basic Science

Abstract

Purpose

In cases of severe retinal diseases, the vitreous body has to be removed and replaced by a suitable biomaterial. Currently, however, no satisfying long-term vitreous substitute is in clinical use. A novel therapeutic concept represents the combination of hyalocytes with suitable biomaterials. The goal of the present study was to evaluate the potential of bFGF and TGF-β1 as tools to control hyalocyte proliferation and the accumulation of extracellular matrix (ECM).

Methods

In vitro investigation on the influence of different concentrations of bFGF and TGF-β1 on hyalocyte morphology, proliferation and ECM production.

Results

Both growth factors affected hyalocyte morphology; small, round cells could be observed after bFGF supplementation, whereas the cells appeared more completely spread when cultured with TGF-β1. Hyalocyte proliferation was increased 3-fold by 10 ng/ml bFGF; 1 ng/ml TGF-β1 in contrast reduced cell proliferation to about 40% of the control. Converse effects of the growth factors could also be observed on the ECM accumulation of hyalocytes; whereas bFGF halved ECM accumulation, TGF-β1 enhanced the ECM production up to 3-fold. Precultivation of hyalocytes with bFGF for two passages had no influence on their subsequent accumulation of glycosaminoglycans (GAG). However, cells precultivated with bFGF exhibited a doubled accumulation of collagen compared to controls.

Conclusions

The observed opposite effects of bFGF and TGF-β1 on hyalocyte proliferation and ECM accumulation may allow for the control of hyaloycte properties. Therefore, these two growth factors seem to be valuable tools towards the development of a cell-based vitreous substitute.

Keywords

Hyalocyte Transforming growth factor beta (TGF-β) Basic fibroblast growth factor (bFGF) Cell-based vitreous substitute Tissue engineering 

Notes

Acknowledgement

This work was financially supported by grant 616/04 from the “Bayerische Forschungsstiftung”, Bavaria, Germany.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Florian Sommer
    • 1
  • Klaus Pollinger
    • 1
  • Ferdinand Brandl
    • 1
  • Barbara Weiser
    • 1
  • Jörg Teßmar
    • 1
  • Torsten Blunk
    • 1
  • Achim Göpferich
    • 1
  1. 1.Department of Pharmaceutical Technology, Institute of PharmacyUniversity of RegensburgRegensburgGermany

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