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Reelin expression is upregulated following ocular tissue injury

  • Jose S. Pulido
  • Ikuko Sugaya
  • Jordan Comstock
  • Kiminobu Sugaya
Short Communication

Abstract

Purpose

Reelin is important in the guidance of neuronal stem cells in the central nervous system during normal development. We wished to determine whether reelin is expressed in the retina and cornea after injury.

Methods

Mice underwent laceration of their retina as well as corneal epithelial debridement. The mice were sacrificed at 3 days, and eyes were fixed and stained for reelin expression and reelin messenger ribonucleic acid (mRNA).

Results

In normal eyes, reelin was expressed only at very low levels in the ganglion cell layer of the retina and the endothelial cell layer of the cornea. In injured eyes, there was marked expression in reelin immunoreactivity in the retina and cornea. Reelin gene expression was seen in the retina and cornea.

Conclusions

Reelin is expressed during normal retinogenesis. This study shows that reelin is also upregulated following injury to the retina and cornea. The expression of reelin following injury suggests that reelin may play an important role in regulating stem cell trafficking in neuronal and nonneuronal tissues following injury similar to its role in normal organogenesis.

Keywords

Cornea Migration Regeneration Retina Stem cell Reelin 

Notes

Acknowledgements

This study was partially supported by an unrestricted grant from Research to Prevent Blindness (University of Illinois, Chicago and Mayo Clinic, Rochester), New York, a Core Grant from the National Eye Institute (EY01792), a grant from the McGraw Foundation, National Institute on Aging (AG 23472), and a grant from Mrs. Charlotte Simmons. We also are grateful to Ms. Christine Pulido and Mr. Andrew Sugaya for their assistance in the experimental aspects of the study.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Jose S. Pulido
    • 1
    • 4
  • Ikuko Sugaya
    • 3
  • Jordan Comstock
    • 2
  • Kiminobu Sugaya
    • 1
    • 2
    • 3
  1. 1.Department of Ophthalmology and Visual SciencesUniversity of Illinois at ChicagoChicagoUSA
  2. 2.Department of PsychiatryUniversity of Illinois at ChicagoChicagoUSA
  3. 3.Biomolecular Science CenterUniversity of Central FloridaOrlandoUSA
  4. 4.Department of OphthalmologyMayo ClinicRochesterUSA

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