Reelin expression is upregulated following ocular tissue injury

  • Jose S. Pulido
  • Ikuko Sugaya
  • Jordan Comstock
  • Kiminobu SugayaEmail author
Short Communication



Reelin is important in the guidance of neuronal stem cells in the central nervous system during normal development. We wished to determine whether reelin is expressed in the retina and cornea after injury.


Mice underwent laceration of their retina as well as corneal epithelial debridement. The mice were sacrificed at 3 days, and eyes were fixed and stained for reelin expression and reelin messenger ribonucleic acid (mRNA).


In normal eyes, reelin was expressed only at very low levels in the ganglion cell layer of the retina and the endothelial cell layer of the cornea. In injured eyes, there was marked expression in reelin immunoreactivity in the retina and cornea. Reelin gene expression was seen in the retina and cornea.


Reelin is expressed during normal retinogenesis. This study shows that reelin is also upregulated following injury to the retina and cornea. The expression of reelin following injury suggests that reelin may play an important role in regulating stem cell trafficking in neuronal and nonneuronal tissues following injury similar to its role in normal organogenesis.


Cornea Migration Regeneration Retina Stem cell Reelin 



This study was partially supported by an unrestricted grant from Research to Prevent Blindness (University of Illinois, Chicago and Mayo Clinic, Rochester), New York, a Core Grant from the National Eye Institute (EY01792), a grant from the McGraw Foundation, National Institute on Aging (AG 23472), and a grant from Mrs. Charlotte Simmons. We also are grateful to Ms. Christine Pulido and Mr. Andrew Sugaya for their assistance in the experimental aspects of the study.


  1. 1.
    Abraham H, Meyer G (2003) Reelin-expressing neurons in the postnatal and adult human hippocampal formation. Hippocampus 13:715–727PubMedCrossRefGoogle Scholar
  2. 2.
    Alcantara S, Ruiz M, D’Arcangelo G, Ezan F, de Lecea L, Curran T, Sotelo C, Soriano E (1998) Regional and cellular patterns of reelin mRNA expression in the forebrain of the developing and adult mouse. J Neurosci 18:7779–7799PubMedGoogle Scholar
  3. 3.
    D’Arcangelo G, Miao GG, Chen SC, Soares HD, Morgan JI, Curran T (1995) A protein related to extracellular matrix proteins deleted in the mouse mutant reeler. Nature 374:719–723PubMedCrossRefGoogle Scholar
  4. 4.
    de Bergeyck V, Naerhuyzen B, Goffinet AM, Lambert de Rouvroit C (1998) A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice. J Neurosci Methods 82:17–24PubMedCrossRefGoogle Scholar
  5. 5.
    Dong X, Pulido JS, Qu T, Sugaya K (2003) Differentiation of human neural stem cells into retinal cells. Neuroreport 14:143–146PubMedCrossRefGoogle Scholar
  6. 6.
    Kim HM, Qu T, Kriho V, Lacor P, Smalheiser N, Pappas GD, Guidotti A, Costa E, Sugaya K (2002) Reelin function in neural stem cell biology. Proc Natl Acad Sci USA 99:4020–4025PubMedCrossRefGoogle Scholar
  7. 7.
    Leavitt BR, Hernit-Grant CS, Macklis JD (1999) Mature astrocytes transform into transitional radial glia within adult mouse neocortex that supports directed migration of transplanted immature neurons. Exp Neurol 157:43–57PubMedCrossRefGoogle Scholar
  8. 8.
    Qu T, Brannen CL, Kim HM, Sugaya K (2001) Human neural stem cells improve cognitive function of aged brain. Neuroreport 12:1127–1132PubMedCrossRefGoogle Scholar
  9. 9.
    Smalheiser NR, Costa E, Guidotti A, Impagnatiello F, Auta J, Lacor P, Kriho V, Pappas GD (2000) Expression of reelin in adult mammalian blood, liver, pituitary pars intermedia, and adrenal chromaffin cells. Proc Natl Acad Sci USA 97:1281–1286PubMedCrossRefGoogle Scholar
  10. 10.
    Wolosin JM, Xiong X, Schutte M, Stegman Z, Tieng A (2000) Stem cells and differentiation stages in the limbo-corneal epithelium. Prog Retin Eye Res 19:223–255PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Jose S. Pulido
    • 1
    • 4
  • Ikuko Sugaya
    • 3
  • Jordan Comstock
    • 2
  • Kiminobu Sugaya
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Ophthalmology and Visual SciencesUniversity of Illinois at ChicagoChicagoUSA
  2. 2.Department of PsychiatryUniversity of Illinois at ChicagoChicagoUSA
  3. 3.Biomolecular Science CenterUniversity of Central FloridaOrlandoUSA
  4. 4.Department of OphthalmologyMayo ClinicRochesterUSA

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