Concentration of neuron-specific enolase and S100 protein in the subretinal fluid of rhegmatogenous retinal detachment
- 84 Downloads
Neuron-specific enolase and S100 protein are markers of neuronal lysis. To assess the neuronal suffering in rhegmatogenous retinal detachment we quantified neuron-specific enolase and S100 protein in the subretinal fluid.
The puncture was performed in the sclera with a Merseture 5/0 round needle, and the fluid was collected with a glass capillary tube. Twelve subretinal fluid samples were obtained from 12 eyes with rhegmatogenous retinal detachment undergoing retinal detachment surgery. Vitreous from ten eyes with macular hole or epimacular membrane served as negative control group, and vitreous collected during cornea procurement from ten deceased patients served as positive control group.
The mean concentration of neuron-specific enolase (in nanogrammes per millilitre) was 602 in the subretinal fluid of rhegmatogenous retinal detachment, 10.2 in the serum of these patients, 2.9 in the vitreous of the negative control group, and 364 in the positive control group. The mean concentration of S100 protein (in nanogrammes per millilitre) was 104 in the subretinal fluid of rhegmatogenous retinal detachment, <0.1 in the serum of these patients and in the vitreous of the control negative group, and 11.18 in the positive control group.
Neuron-specific enolase (NSE) and S100 are known to be good markers of brain stress and, thus, are good markers of retinal stress.
KeywordsBiology NSE Retinal detachment S100 protein Subretinal fluid
- 8.Coquerel A, Loeb A, Proust B, Jeannot E, Fessard C (1989) An index of neuronal lysis: neuron-specific enolase (NSE) in cerebrospinal fluid; diagnostic and prognostic values in newborns and infants. Biologie prospective. In: Galeau MM, Siest G, Henry J (eds) Comptes rendus du 7ème colloque de Pont-à-Mousson. John Libbey Eurotext, Paris, pp 253–256Google Scholar
- 9.De Bokay E, Aouididi S, Coquerel A (1995) Subretinal fluid elevation of NSE in patients with retinal detachment. Investig Ophthalmol Vis Sci 36:S7001Google Scholar
- 13.Keating Mac EG, Andrews PJ, Mascia L (1998) Relationship of neuron specific enolase and protein S-100 concentrations in systemic and jugular venous serum to injury severity and outcome after traumatic brain injury. Acta Neurochir Suppl (Wien) 71:117–119Google Scholar
- 22.Sebag J, Tuyen V, Faure JP, Chauvaud D, Pouliquen Y (1987) Retinal S-antigen in human subretinal fluid. Investig Ophthalmol Vis Sci 28(12):2038–2041Google Scholar
- 27.Steinhoff BJ, Tumani H, Otto M, Mursch K, Wiltfang J, Herrendorf G, Bittermmann HJ, Felgenhauser K, Paulus W, Markakis E (1999) Cisternal S100 protein and neuron-specific enolase are elevated and site-specific markers in intractable temporal lobe epilepsy. Epilepsy Res 36(1):75–82CrossRefPubMedGoogle Scholar
- 29.Zimmer DB, Eldik van LJ (1987) Tissue distribution of rat S100α and S100β and S100-binding proteins. Am J Physiol Soc 252(3Pt 1):C285–C289Google Scholar