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Influence of a new surface modification of intraocular lenses with fluoroalkylsilan on the adherence of endophthalmitis-causing bacteria in vitro

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Abstract

Introduction

Dynasilan is a fluoroalkylsilan that is able to interact with surface active centres on intraocular lenses (IOL), offering a new way for surface modification of different IOL materials. The purpose of this in vitro study was to investigate the influence of this new surface modification on the adherence of two typical endophthalmitis causing bacteria (Staphylococcus epidermidis, Propionibacterium acnes).

Materials and methods

In a pilot experiment, the effect of Dynasilan coating on the adherence of S. epidermidis was tested on glass slides. Forty-two Dynasilan-modified and 42 unmodified IOL (14 PMMA, 14 silicone and 14 hydrogel) were incubated at 37°C in brain heart infusion broth (108 CFU/ml) with either S. epidermidis for 24 h or with P. acnes for 1 h. Subsequently, the adherent bacteria were resuspended using ultrasonification at 35 kHz for 3×45 s. After dilution series and incubation at 37°C on Petri dishes for 24 h and 3 days, respectively, the colonies were counted.

Results

In the pilot experiment, a markedly lower number of adherent S. epidermidis was observed on Dynasilan-modified glass slides. Of all IOL materials incubated with S. epidermidis, those modified with Dynasilan showed a lower mean number of adherent bacteria (mean 1.37×107; SD 2.37×107) than those untreated (2.43×107; SD 3.04×107).

IOLs incubated with P. acnes showed a significantly lower mean number of adherent bacteria of 2.51×104 (SD 2.71×104) on Dynasilan-modified IOLs versus 6.27×104 (SD 7.70×104) on untreated IOLs.

Conclusion

The presented in vitro results indicate that Dynasilan surface modification is able to reduce the adherence of S. epidermidis and P. acnes on all IOL materials tested.

Further studies regarding the stability of this modification and its biocompatibility must be performed.

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Authors and Affiliations

  1. University Eye Clinic Lübeck, Lübeck, Germany

    Antonia Kienast, Horst Laqua & Hans Hoerauf

  2. Institute of Hygiene, University Lübeck, Lübeck, Germany

    Regine Kämmerer, Helge Ohgke & Werner Solbach

  3. Institute of Medical Statistics, University Aachen, Aachen, Germany

    Claudia Weiss

  4. Institute of Anatomy, University Lübeck, Lübeck, Germany

    Matthias Klinger

  5. PharmPur GmbH, Augsburg, Germany

    Dirk-Henning Menz

  6. Bausch & Lomb Surgical GmbH, München, Germany

    Joachim Dresp

  7. Klinik für Augenheilkunde, Universitätsklinikum Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany

    Hans Hoerauf

Authors
  1. Antonia Kienast
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  2. Regine Kämmerer
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  3. Claudia Weiss
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  4. Matthias Klinger
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  5. Dirk-Henning Menz
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  6. Joachim Dresp
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  7. Helge Ohgke
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  8. Werner Solbach
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  9. Horst Laqua
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  10. Hans Hoerauf
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Correspondence to Hans Hoerauf.

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Kienast, A., Kämmerer, R., Weiss, C. et al. Influence of a new surface modification of intraocular lenses with fluoroalkylsilan on the adherence of endophthalmitis-causing bacteria in vitro. Graefe's Arch Clin Exp Ophthalmo 244, 1171–1177 (2006). https://doi.org/10.1007/s00417-005-0242-x

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  • DOI: https://doi.org/10.1007/s00417-005-0242-x

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