Non-invasive polarimetric measurement of glucose concentration in the anterior chamber of the eye

  • Rainer Rawer
  • Wilhelm Stork
  • Cristine F. Kreiner
Short Communication



Diabetes mellitus is one of the most common diseases in industrialized countries as well as in emerging economies such as India or China. One of the key technologies for diabetes therapy is semi-continuous monitoring of the glucose level of diabetics.


Compared with skin-perforating techniques, optical measurement techniques promising good results bear the potential for high patient compliance with more frequent measurements. Due to its excellent optical properties, the anterior chamber and the aqueous humor (AH) contained therein offer promise for non-invasive in vivo glucose measurements. However, a number of strongly limiting factors, such as the precise optical properties of the eye, laser safety regulations and subconscious eye movements during the measurement period have to be considered for in vivo applications.


This article presents a high-resolution polarimetric measurement system that utilizes the optical rotatory dispersion (optical activity) of the glucose molecule for measurements of the glucose concentration in AH.


Based on this example of a suitable optical measurement system, the special limitations and conditions that have to be considered for in vivo glucose measurement at the human eye are presented and analyzed. This includes the optical properties of the cornea and the anterior chamber, the impact of typical eye movements during a measurement and laser safety regulations.


Anterior Chamber Aqueous Humor Polarization Plane Optical Rotatory Dispersion Optical Measurement System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was partially supported by grants from the VDI/VDE-Technologiezentrum Informationstechnik (VDI/VDE-IT) on behalf of the German Ministry for Research and Education (BMBF) program “Microsystemtechnik 2000+.”


  1. 1.
    Alio J, Pradon M (1982) Influence of age on temperature of the anterior segment of the eye. Measurement by infrared thermometry. Ophthalmic Res 14:153–159PubMedGoogle Scholar
  2. 2.
    Baba J, Meledeo A, Cameron BD, Coté GL (2001) Investigation of pH and temperature on optical rotatory dispersion for noninvasive glucose monitoring. Proc SPIE 4263Google Scholar
  3. 3.
    Böckle S, Rovati L, Ansari RR (2002) Polarimetric glucose sensing using Brewster-reflection off of eye lens: theoretical analysis, optical diagnostics and sensing of biological fluids and glucose and cholesterol monitoring II. Proc SPIE 4624:160–164Google Scholar
  4. 4.
    Born M, Wolf E (1980) Principles of optics. Pergamon, New YorkGoogle Scholar
  5. 5.
    Cameron BD, Coté GL (2000) Optical polarimetry applied to the development of a noninvasive in-vivo glucose monitor. Proc SPIE BiOSGoogle Scholar
  6. 6.
    Cameron BD, Gorde H, Coté GL (1999) Development of an optical polarimeter for in vivo glucose monitoring. SPIE 3599:43–49CrossRefGoogle Scholar
  7. 7.
    Center for Disease Control:, National diabetics fact sheet
  8. 8.
    Chou C, Lin P-K (2000) Noninvasive glucose monitoring with optical heterodyne technique. Diabetes Technol Ther 2:45–47CrossRefPubMedGoogle Scholar
  9. 9.
    Diabetes Control and Complications Trial Research Group (1993) The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. N Engl J Med 329:977–986CrossRefPubMedGoogle Scholar
  10. 10.
    Diabetes Control and Complications Trial Research Group (1997) Lifetime benefits and costs of intensive therapy as practiced in the diabetes control and complications trial. Clin Diabetes 15:140–146Google Scholar
  11. 11.
    European safety laser regulations: EN/IEC 60825-1:1993 + A11:1996 + A2:2001 (November 2001)Google Scholar
  12. 12.
    Gullstrand A (1924) Helmholz’s physiological optics. Optical Society of America, App. pp 350–358Google Scholar
  13. 13.
    Liou H-L, Brennan NA (1997) Anatomically accurate, finite eye model for optical modelling. J Opt Soc Am A 14:1684–1695PubMedGoogle Scholar
  14. 14.
    International Diabetes Foundation:
  15. 15.
    Le Grand Y, El Hage SG (1980) Physiological optics. Springer Series in Optical Science. Springer, Berlin Heidelberg New YorkGoogle Scholar
  16. 16.
    McNichols RJ, Cameron BD, Coté GL (2001) Development of a non-invasive polarimetric glucose sensor, IEEE, LEOS NewsletterGoogle Scholar
  17. 17.
    Pierscionek BK (1994) Refractive index of the human lens surface measured with an optical fibre sensor. Ophthalmic Res 66:32–35Google Scholar
  18. 18.
    Pierscionek BK, Chan DYC (1998) Refractive index gradient of human lenses. Optom Vis Sci 66:822–829Google Scholar
  19. 19.
    Rawer R, Stork W et al (2002) Polarimetric methods for measurements of intra ocular glucose concentration. 36. Jahrestagung der Deutschen Gesellschaft für Biomedizinische Technik (DGBMT). Schiele Schön 47:186–187Google Scholar
  20. 20.
    Rawer R, Malz A, Vollmer P, Stork W (2003) Analysis of signal processing scheme for polarimetric in-vivo glucose measurement in aqueous humor. 37. Jahrestagung der Deutschen Gesellschaft für Biomedizinische Technik (DGBMT). Schiele Schön 48:430–431Google Scholar
  21. 21.
    Rysa P, Sarvaranta J (1974) Corneal temperature in man and rabit. Observations made using an infra-red camera and a cold chamber. Acta Ophthalmol Copenh Suppl 123:324–339Google Scholar
  22. 22.
    Schrader W (2000) Spektrometrie des Auges: Wege zur nichtinvasiven Diagnostik. Habilitationsschrift, Julius-Maximilians Universität WürzburgGoogle Scholar
  23. 23.
    World Health Organization (WHO)
  24. 24.
    Yu NT, Long JR, Price JF et al (1996) Development of a noninvasive diabetes screening device using the ratio of fluorescence to Rayleigh scattered light. J Biomed Opt 3:280–288Google Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Rainer Rawer
    • 1
  • Wilhelm Stork
    • 1
  • Cristine F. Kreiner
    • 2
  1. 1.Institut für Technik der Informationsverarbeitung (ITIV)Universität KarlsruheKarlsruheGermany
  2. 2.Acri.Tec GmbHGlienicke, BerlinGermany

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