Expression of estrogen receptor α and 17β-hydroxysteroid dehydrogenase 4 in the ciliary body

  • Kaori Kobayashi
  • Ryo Iwakiri
  • Hiroshi Kobayashi
  • Satoshi Okinami
Laboratory Investigation
First Online:
Received:
Revised:
Accepted:

Abstract

Purpose

To investigate the expression and distribution of estrogen receptor α (ERα) and 17β-hydroxysteroid dehydrogenase 4 (HSD4) in bovine and monkey ciliary body.

Methods

Immunohistochemical study was employed to investigate the expression of ERα and 17β-HSD4. To detect ERα and 17β-HSD4 transcripts, reverse transcription–polymerase chain reaction (RT-PCR) was used.

Results

In bovine and monkey ciliary body, the staining of the anti-ERα antibody was distributed in nonpigmented epithelium, and was not detectable in pigmented epithelium. Vessels in the stroma were also stained. The expression of 17β-HSD4 was observed in nonpigmented and pigmented epithelium. RT-PCR revealed that the transcripts of ERα and 17β-HSD4 were expressed in monkey ciliary body.

Conclusions

This study provides evidence for the presence of ERα and 17β- HSD4 in the ciliary body. The presence of ERα and 17β-HSD4 suggests that estrogen may have important functions in the ciliary body.

Keywords

Estrogen Receptor Retinal Pigment Epithelium Intraocular Pressure Pigment Epithelium Aqueous Humor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Kaori Kobayashi
    • 1
  • Ryo Iwakiri
    • 1
  • Hiroshi Kobayashi
    • 1
  • Satoshi Okinami
    • 1
  1. 1.Department of OphthalmologySaga Medical SchoolSaga Japan

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