Journal of Neurology

, Volume 245, Issue 12, pp 781–793

Histological investigation of spinal cord lesions in the spinal hyperostotic mouse (twy/twy): morphological changes in anterior horn cells and immunoreactivity to neurotropic factors

  • K. Uchida
  • Hisatoshi Baba
  • Yasuhisa Maezawa
  • Shoei Furukawa
  • Nobuaki Furusawa
  • Shinichi Imura
Original communication

DOI: 10.1007/s004150050287

Cite this article as:
Uchida, K., Baba, H., Maezawa, Y. et al. J Neurol (1998) 245: 781. doi:10.1007/s004150050287

Abstract

We examined the morphology of spinal accessory motoneurons and immunoreactivity to neurotrophins, brain-derived neurotropic factor (BDNF) and neurotrophin (NT)-3, as well as the presence of reactive astrocytosis in 70 tiptoe walking Yoshimura (twy) mice that develop calcification at C1-C2 vertebral level compressing the spinal cord. At the level of compression, the area of neuronal soma and total length of dendrites of wheat germ agglutinin-horseradish peroxidase (WGA-HRP)-labelled accessory motoneurons in the medial cell pool decreased significantly with decrement in motoneuron population, relative to the control. In contrast, at sites rostral to the compressive lesion, a significant enlargement of the neuron soma and dendritic elongation were noted, associated with increased motoneuron population and decreased transverse area of the cord at the level of compression. At this site, enhanced BDNF and NT-3 immunoreactivities were evident in the anterior horn cells. In mice with a more severe degree of compression, astrocyte-like cells showing BDNF immunoreactivity became abundant and axons in the anterior column demonstrated a marked NT-3 immunoreactivity. Our results suggest increased functional activity of anterior horn cells at levels rostral to the site of compression. We speculate that the presence of BDNF and NT-3 in neurons and astrocyte-like cells is proportionate to the severity of chronic mechanical compression and may contribute to the heterotropic neuronal reserve and survival.

Key words Spinal accessory motoneuron Anterior horn cell Neurotrophins Wheat germ agglutinin-horseradish peroxidase Tiptoe-walking Yoshimura (twy) mouse 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • K. Uchida
    • 1
  • Hisatoshi Baba
    • 1
  • Yasuhisa Maezawa
    • 1
  • Shoei Furukawa
    • 2
  • Nobuaki Furusawa
    • 1
  • Shinichi Imura
    • 1
  1. 1.Department of Orthopaedic Surgery, School of Medicine, Fukui Medical University, Shimoaizuki 23, Matsuoka, Fukui 910-1193, Japan e-mail: baba@fmsrsa.fukui-med.ac.jp Tel.: +81-776-61-3111, ext. 2354/2356 Fax: +81-776-61-8125JP
  2. 2.Laboratory of Molecular Biology, Gifu Pharmaceutical University, Mitahora-higashi, Gifu 502-0002, JapanJP

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