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Journal of Neurology

, Volume 258, Issue 10, pp 1781–1787 | Cite as

Multiplex PCR for rapid diagnosis of tuberculous meningitis

  • Sharma Kusum
  • Sharma Aman
  • Ray Pallab
  • Sharma Shiv Kumar
  • Modi Manish
  • Prabhakar Sudesh
  • Varma Subhash
  • Sharma Meera
Original Communication

Abstract

Rapid and specific diagnosis of tubercular meningitis is of paramount importance to decrease morbidity and mortality. The aim of the study was to evaluate multiplex PCR using protein b, MPB 64, and IS6110 primers directed against M. tuberculosis complex for the diagnosis of tuberculous meningitis (TBM). Multiplex PCR was performed on 18 TBM confirmed cases (culture was positive), 92 clinically suspected TBM cases and 100 non-TBM (control group) patients. Multiplex PCR had a sensitivity of 94.4% for confirmed cases and specificity of 100% for confirmed TBM cases. In 92 clinically diagnosed but unconfirmed TBM cases, multiplex PCR was positive in 84.78% cases. The overall sensitivity of microscopy, culture and multiplex cases were 1.81, 16.73, and 86.63% and specificity was 100, 100, and 100% respectively. Multiplex PCR using protein b, MPB 64, and IS6110 primers has a high sensitivity and specificity in diagnosis of tubercular meningitis.

Keywords

Tuberculous meningitis PCR Multiplex PCR Molecular diagnosis 

Notes

Conflict of interest

None.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Sharma Kusum
    • 1
  • Sharma Aman
    • 2
  • Ray Pallab
    • 1
  • Sharma Shiv Kumar
    • 1
  • Modi Manish
    • 3
  • Prabhakar Sudesh
    • 3
  • Varma Subhash
    • 2
  • Sharma Meera
    • 1
  1. 1.Department of Medical MicrobiologyPost Graduate Institute of Medical Education and ResearchChandigarhIndia
  2. 2.Department of Internal MedicinePost Graduate Institute of Medical Education and ResearchChandigarhIndia
  3. 3.Department of NeurologyPost Graduate Institute of Medical Education and ResearchChandigarhIndia

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