Journal of Neurology

, Volume 258, Issue 2, pp 255–262

Tau and 14-3-3 of genetic and sporadic Creutzfeldt–Jakob disease patients in Israel

  • Zeev Meiner
  • Esther Kahana
  • Fanny Baitcher
  • Amos D. Korczyn
  • Joab Chapman
  • Oren S. Cohen
  • Ron Milo
  • Judith Aharon-Perez
  • Oded Abramsky
  • Ruth Gabizon
  • Hanna Rosenmann
Original Communication

Abstract

One of the largest clusters of genetic Creutzfeldt–Jakob disease (gCJD) is found among Jews of Libyan origin in Israel and is linked to the E200K mutation in PRNP (gCJDE200K). The aim of this study was to compare the levels of cerebrospinal fluid (CSF) biomarkers, Tau and 14-3-3 proteins, between gCJDE200K patients, sporadic CJD (sCJD) patients and non-CJD controls in Israel between the years 1996–2006. The levels of Tau and 14-3-3 proteins in CSF were measured by ELISA and immunoblotting, respectively. CSF Tau levels were similar in gCJDE200K and sCJD, both were significantly higher than in controls [1,107 ± 470 pg/ml [33/46 (72%)] of the cases >1,000 pg/ml, 1,280 ± 580 pg/ml [25/30 (83.3%)], and 354 ± 338 pg/ml [17/243 (6.9%)], respectively, p < 0.001]. 14-3-3 was detected in CSF of 41/53 (77%) of each gCJDE200K and sCJD patients tested, but only in 70/417 (16.8%) of controls (p < 0.001). An inverse correlation was found between disease duration and Tau levels in both gCJDE200K and sCJD (r = −0.464 and r = −0.284). No difference was found in Tau or 14-3-3 between the various codon 129 genotypes. We conclude that CSF biomarkers, Tau and 14-3-3, may be used in the diagnosis in both patients’ populations, presenting a similar sensitivity yet Tau assay having higher specificity.

Keywords

Tau 14-3-3 protein Cerebrospinal fluid Creutzfeldt–Jakob disease Libyan Jews 

References

  1. 1.
    Alperovitch A, Zerr I, Pocchiari M, Mitrova E, de Pedro Cuesta J, Hegyi I, Collins S, Kretzschmar H, van Duijn C, Will RG (1999) Codon 129 prion protein genotype and sporadic Creutzfeldt–Jakob disease. Lancet 353:1673–1674CrossRefPubMedGoogle Scholar
  2. 2.
    Buerger K, Otto M, Teipel SJ, Zinkowski R, Blennow K, DeBernardis J, Kerkman D, Schröder J, Schönknecht P, Cepek L, McCulloch C, Möller HJ, Wiltfang J, Kretzschmar H, Hampel H (2006) Dissociation between CSF total Tau and Tau protein phosphorylated at threonine 231 in Creutzfeldt-Jakob disease. Neurobiol Aging 27:10–5Google Scholar
  3. 3.
    Castellani RJ, Colucci M, Xie Z, Zou W, Li C, Parchi P, Capellari S, Pastore M, Rahbar MH, Chen SG, Gambetti P (2004) Sensitivity of 14-3-3 protein test varies in subtypes of sporadic Creutzfeldt-Jakob disease. Neurology 63:436–442PubMedGoogle Scholar
  4. 4.
    Cepek L, Steinacker P, Mollenhauer B, Wiese B, Ciesielczyk B, Bibl M, Wiltfang J, Zerr I, Schulz-Schaeffer W, Kretzschmar HA, Poser S, Otto M (2005) Follow-up investigations of Tau protein and S-100B levels in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. Dement Geriatr Cogn Disord 19:376–382CrossRefPubMedGoogle Scholar
  5. 5.
    Chapman J, Korczyn AD (1991) Genetic and environmental factors determining the development of Creutzfeldt-Jakob disease in Libyan Jews. Neuroepidemiology 10:228–231CrossRefPubMedGoogle Scholar
  6. 6.
    Gabizon R, Rosenmann H, Meiner Z, Kahana I, Kahana E, Shugart Y, Ott J, Prusiner SB (1993) Mutation and polymorphism of the prion protein gene in Libyan Jews with Creutzfeldt-Jakob disease (CJD). Am J Hum Genet 53:828–835PubMedGoogle Scholar
  7. 7.
    Geschwind MD, Martindale J, Miller D, DeArmond SJ, Uyehara-Lock J, Gaskin D, Kramer JH, Barbaro NM, Miller BL (2003) Challenging the clinical utility of the 14-3-3 protein for the diagnosis of sporadic Creutzfeldt-Jakob disease. Arch Neurol 60:813–816CrossRefPubMedGoogle Scholar
  8. 8.
    Glodzik-Sobanska L, Pirraglia E, Brys M, de Santi S, Mosconi L, Rich KE, Switalski R, Saint Louis L, Sadowski MJ, Martiniuk F, Mehta P, Pratico D, Zinkowski RP, Blennow K, de Leon MJ (2009) The effects of normal aging and ApoE genotype on the levels of CSF biomarkers for Alzheimer’s disease. Neurobiol Aging 30:672–681CrossRefPubMedGoogle Scholar
  9. 9.
    Goldfarb LG, Korczyn AD, Brown P, Chapman J, Gajdusek DC (1990) Mutation in codon 200 of scrapie amyloid precursor gene linked to Creutzfeldt-Jakob disease in Sephardic Jews of Libyan and non-Libyan origin. Lancet 336:637–638CrossRefPubMedGoogle Scholar
  10. 10.
    Green AJ, Thompson EJ, Stewart GE, Zeidler M, McKenzie JM, MacLeod MA, Ironside JW, Will RG, Knight RS (2001) Use of 14-3-3 and other brain-specific proteins in CSF in the diagnosis of variant Creutzfeldt-Jakob disease. J Neurol Neurosurg Psychiatry 70:744–748CrossRefPubMedGoogle Scholar
  11. 11.
    Green AJ, Ramljak S, Muller WE, Knight RS, Schroder HC (2002) 14-3-3 in the cerebrospinal fluid of patients with variant and sporadic Creutzfeldt-Jakob disease measured using capture assay able to detect low levels of 14-3-3 protein. Neurosci Lett 324:57–60CrossRefPubMedGoogle Scholar
  12. 12.
    Green A, Sanchez-Juan P, Ladogana A, Cuadrado-Corrales N, Sánchez-Valle R, Mitrová E, Stoeck K, Sklaviadis T, Kulczycki J, Heinemann U, Hess K, Slivarichová D, Saiz A, Calero M, Mellina V, Knight R, van Duijn CM, Zerr I (2007) CSF analysis in patients with sporadic CJD and other transmissible spongiform encephalopathies. Eur J Neurol 14:121–124CrossRefPubMedGoogle Scholar
  13. 13.
    Hsiao K, Meiner Z, Kahana E, Cass C, Kahana I, Avrahami D, Scarlato G, Abramsky O, Prusiner SB, Gabizon R (1991) Prion protein mutation in Libyan Jews with Creutzfeldt-Jakob disease. N Eng J Med 324:1091–1097CrossRefGoogle Scholar
  14. 14.
    Hsiech G, Kenney K, Gibbs CJ Jr, Lee KH, Harrington MG (1996) The 14-3-3 brain protein in cerebrospinal fluid as a marker for transmissible spongiform encephalopathies. N Engl J Med 335:924–930CrossRefGoogle Scholar
  15. 15.
    Kahana E, Alter M, Braham J, Sofer D (1974) Creutzfeldt-Jakob disease: focus among Libyan Jews in Israel. Science 183:90–91CrossRefPubMedGoogle Scholar
  16. 16.
    Kahana E, Zilber N, Abraham M (1991) Do Creutzfeldt-Jakob disease patients of Jewish Libyan origin have unique clinical features? Neurology 41:1390–1392PubMedGoogle Scholar
  17. 17.
    Korczyn AD (1991) Creutzfeldt-Jakob disease among Libyan Jews. Eur J Epidemiol 7:490–493CrossRefPubMedGoogle Scholar
  18. 18.
    Kovács GG, Puopolo M, Ladogana A, Pocchiari M, Budka H, van Duijn C, Collins SJ, Boyd A, Giulivi A, Coulthart M, Delasnerie-Laupretre N, Brandel JP, Zerr I, Kretzschmar HA, de Pedro-Cuesta J, Calero-Lara M, Glatzel M, Aguzzi A, Bishop M, Knight R, Belay G, Will R, Mitrova E (2005) Genetic prion disease: the EUROCJD experience. Hum Genet 118:166–174CrossRefPubMedGoogle Scholar
  19. 19.
    Ladogana A, Sanchez-Juan P, Mitrová E, Green A, Cuadrado-Corrales N, Sánchez-Valle R, Koscova S, Aguzzi A, Sklaviadis T, Kulczycki J, Gawinecka J, Saiz A, Calero M, van Duijn CM, Pocchiari M, Knight R, Zerr I (2009) Cerebrospinal fluid biomarkers in human genetic transmissible spongiform encephalopathies. J Neurol 256:1620–1628CrossRefPubMedGoogle Scholar
  20. 20.
    Mead S (2006) Prion disease genetics. Eur J Hum Genet 14:273–281CrossRefPubMedGoogle Scholar
  21. 21.
    Meiner Z, Gabizon R, Prusiner SB (1997) Inherited Creutzfeldt-Jakob disease in Libyan Jews caused by mutation at codon 200 in the prion protein gene. Medicine (Baltimore) 76:227–237CrossRefGoogle Scholar
  22. 22.
    Meissner B, Körtner K, Bartl M, Jastrow U, Mollenhauer B, Schröter A, Finkenstaedt M, Windl O, Poser S, Kretzschmar HA, Zerr I (2004) Sporadic Creutzfeldt-Jakob disease: magnetic resonance imaging and clinical findings. Neurology 63:450–456PubMedGoogle Scholar
  23. 23.
    Mitrová E, Belay G (2002) Creutzfeldt-Jakob disease with E200K mutation in Slovakia: characterization and development. Acta Virol 46:31–39PubMedGoogle Scholar
  24. 24.
    Mollenhauer B, Serafin S, Zerr I, Steinhoff BJ, Otto M, Scherer M, Schulz-Schaeffer WJ, Poser S (2003) Diagnostic problems during late course in Creutzfeldt-Jakob disease. J Neurol 250:629–630CrossRefPubMedGoogle Scholar
  25. 25.
    Otto M, Wiltfang J, Tumani H, Zerr I, Lantsch M, Kornhuber J, Weber T, Kretzschmar HA, Poser (1997) Elevated levels of Tau-protein in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. Neurosci Lett 225:210–212CrossRefPubMedGoogle Scholar
  26. 26.
    Otto M, Wiltfang J, Cepek L, Neumann M, Mollenhauer B, Steinacker P, Ciesielczyk B, Schulz-Schaeffer W, Kretzschmar HA, Poser S (2002) Tau protein and 14-3-3 protein in the differential diagnosis of Creutzfeldt-Jakob disease. Neurology 58:192–197PubMedGoogle Scholar
  27. 27.
    Prusiner SB (2001) Shattuck lecture—neurodegenerative diseases and prions. N Engl J Med 344:1516–1526CrossRefPubMedGoogle Scholar
  28. 28.
    Rosenmann H, Meiner Z, Kahana E, Halimi M, Lenetsky E, Abramsky O, Gabizon R (1997) Detection of 14-3-3 protein in the CSF of genetic Creutzfeldt-Jakob disease. Neurology 49:593–595PubMedGoogle Scholar
  29. 29.
    Sanchez-Juan P, Green A, Ladogana A, Cuadrado-Corrales N, Sáanchez-Valle R, Mitrováa E, Stoeck K, Sklaviadis T, Kulczycki J, Hess K, Bodemer M, Slivarichová D, Saiz A, Calero M, Ingrosso L, Knight R, Janssens AC, van Duijn CM, Zerr I (2006) CSF tests in the differential diagnosis of Creutzfeldt-Jakob disease. Neurology 67:637–643CrossRefPubMedGoogle Scholar
  30. 30.
    Sanchez-Juan P, Sánchez-Valle R, Green A, Ladogana A, Cuadrado-Corrales N, Mitrová E, Stoeck K, Sklaviadis T, Kulczycki J, Hess K, Krasnianski A, Equestre M, Slivarichová D, Saiz A, Calero M, Pocchiari M, Knight R, van Duijn CM, Zerr I (2007) Influence of timing on CSF tests value for Creutzfeldt-Jakob disease diagnosis. J Neurol 254:901–906CrossRefPubMedGoogle Scholar
  31. 31.
    Satoh K, Shirabe S, Tsujino A, Eguchi H, Motomura M, Honda H, Tomita I, Satoh A, Tsujihata M, Matsuo H, Nakagawa M, Eguchi K (2007) Total Tau protein in cerebrospinal fluid and diffusion-weighted MRI as an early diagnostic marker for Creutzfeldt-Jakob disease. Dement Geriatr Cogn Disord 24:207–212CrossRefPubMedGoogle Scholar
  32. 32.
    Satoh K, Shirabe S, Eguchi H, Tsujino A, Eguchi K, Satoh A, Tsujihata M, Niwa M, Katamine S, Kurihara S, Matsuo H (2006) 14-3-3 Protein, total Tau and phosphorylated Tau in cerebrospinal fluid of patients with Creutzfeldt-Jakob Disease and neurodegenerative disease in Japan. Cell Mol Neurobiol 26:45–52CrossRefPubMedGoogle Scholar
  33. 33.
    Van Everbroeck B, Quoilin S, Boons J, Martin JJ, Cras P (2003) A prospective study of CSF markers in 250 patients with possible Creutzfeldt-Jakob disease. J Neurol Neurosurg Psychiatry 74:1210–1214CrossRefPubMedGoogle Scholar
  34. 34.
    Will RG (1993) Epidemiology of Creutzfeldt-Jakob disease. Br Med Bull 49:960–970PubMedGoogle Scholar
  35. 35.
    World Health Organization (1998) Consensus on criteria for sporadic CJD. WHO, GenevaGoogle Scholar
  36. 36.
    Young GS, Geschwind MD, Fischbein NJ, Martindale JL, Henry RG, Liu S, Lu Y, Wong S, Liu H, Miller BL, Dillon WP (2005) Diffusion-weighted and fluid-attenuated inversion recovery imaging in Creutzfeldt-Jakob disease: high sensitivity and specificity for diagnosis. AJNR Am J Neuroradiol 26:1551–1562PubMedGoogle Scholar
  37. 37.
    Zerr I, Bodemer M, Gefeller O, Otto M, Poser S, Wiltfang J, Windl O, Kretzschmar HA, Weber T (1998) Detection of 14-3-3 protein in the cerebrospinal fluid supports the diagnosis of Creutzfeldt-Jakob disease. Ann Neurol 43:32–40CrossRefPubMedGoogle Scholar
  38. 38.
    Zilber N, Kahana E, Abraham M (1991) The Libyan Creutzfeldt-Jakob disease focus in Israel: an epidemiologic evaluation. Neurology 41:1385–1389PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Zeev Meiner
    • 1
    • 2
  • Esther Kahana
    • 3
  • Fanny Baitcher
    • 1
  • Amos D. Korczyn
    • 4
  • Joab Chapman
    • 5
  • Oren S. Cohen
    • 5
  • Ron Milo
    • 3
  • Judith Aharon-Perez
    • 6
  • Oded Abramsky
    • 1
  • Ruth Gabizon
    • 1
  • Hanna Rosenmann
    • 1
  1. 1.Departments of NeurologyHadassah University HospitalJerusalemIsrael
  2. 2.Physical Medicine and RehabilitationHadassah University HospitalJerusalemIsrael
  3. 3.Department of NeurologyBarzilai Medical CenterAshkelonIsrael
  4. 4.Sieratzky Chair of NeurologyTel Aviv UniversityTel AvivIsrael
  5. 5.Department of NeurologySheba Medical CenterTel AvivIsrael
  6. 6.Department of NeurologyRambam Medical CenterHaifaIsrael

Personalised recommendations