Neurological and functional recovery in human stroke are associated with peripheral blood CD34+ cell mobilization
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A spontaneous mobilization of Peripheral Blood-Mononuclear CD34+ Cells (PB-MNC-CD34+) has recently been reported in human myocardial infarction and found to be related to improved heart function and survival. However, nothing is known regarding a possible relation between PB-MNC-CD34+ mobilization and neurological recovery in human acute cerebral ischemia.
Methods and Results
PB-MNC-CD34+ were determined daily after an acute cerebral ischemic attack for 14 days in 25 patients with acute ischemic stroke and compared with controls. Results indicated that stroke was followed by large and bursting mobilizations of PB-MNC-CD34+. The amplitude of the mobilizations was similar to those observed in Granulocyte Colony Stimulating Factor (G-CSF) conditioned aplastic patients following myeloablative therapy before leukapheresis and autologous bone graft. The extent of PB-MNC-CD34+ mobilization in each patient was directly related to neurological and functional recoveries as assessed by NIH Stroke Scale, and modified Rankin Scale respectively.
The mobilization of PB-MNC-CD34+ cells might be predictive of neurological and functional recovery.
Keywordsangiogenesis neurological recovery functional recovery stem cell plasticity stroke
Authors are very thankful to: Dr Frederic Berthier (from the “Département d’Information Médicale, CHU de Nice) for his valuable contribution in biostatistics; Mr Julien Nivet, Clinical Research Associate; the National Institute of Science and Medical Research and the University Hospital of Nice. We are also very thankful to the “UEFCT’s technicians”.
- 5.Dunac A, Frelin C, Mahagne MH, Chatel M, Philip PJM (2002) Mobilization of CD34+ progenitor cells during recovery in acute ischemic stroke: a new mechanism for cerebral ischemic tissue repair. J Neurol 249: (S1) I/31Google Scholar
- 16.Murry CE, Soonpaa MH, Reinecke H, Nakajima H, Nakajima HO, Rubart M, Pasumarthi KB, Virag JI, Bartelmez SH, Poppa V, Bradford G, Dowell JD, Williams DA, Field LJ (2004) Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature 428:664–668PubMedCrossRefGoogle Scholar
- 20.Paczkowska E, Larysz B, Rzeuski R, Karbicka A, Jałowinski R, Kornacewicz-Jach Z, Ratajczak MZ, Machalinski B (2005) Human hematopoietic stem/progenitor-enriched CD34+ cells are mobilized into peripheral blood during stress related to ischemic stroke or acute myocardial infarction. Eur J Haematol 75:461–467PubMedCrossRefGoogle Scholar
- 29.Wang Y, Tägil K, Ripa RS, Nilsson JC, Carstensen S, JØrgensen E, SØndergaard L, Hesse B, Johnsen HE, Kastrup J (2005) Effect of mobilization of bone marrow stem cells by granulocyte colony stimulating factor on clinical symptoms, left ventricular perfusion and function in patients with severe chronic ischemic heart disease. Inter J Cardiol 100:477–483CrossRefGoogle Scholar
- 30.Yu J, Leisenring W, Bensinger WI, Holmberg LA, Rowley SD (1999) The predictive value of white cell or CD34 cell counts in the peripheral blood for timing apheresis and maximizing yield. Transplantation 39:442–450Google Scholar