International Journal of Legal Medicine

, Volume 110, Issue 6, pp 312–315 | Cite as

Additional primer sets for an amelogenin gene PCR-based DNA-sex test

  • H. Haas-Rochholz
  • G. Weiler
Original article

Abstract

When aligning the human amelogenin X (AmelX)-gene with the human amelogenin Y (AmelY)-gene, 19 regions of absolute homology ranging in size from 22 to 80 bp, 5 deletions located on AmelX from 1 to 6 bp and 5 deletions 1–183 bp long located on AmelY can be observed. The regions of absolute homology are used to design primer sets which span deletions of the X- and/ or Y-chromosome. The PCR products generated on the X- and Y-homologues differ in size. New regions of deletions and newly designed primers are described to facilitate the integration of the amelogenin sex test into multiplex PCR reactions.

Key words Amelogenin Sex test Deletions PCR Alignment STRs 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • H. Haas-Rochholz
    • 1
  • G. Weiler
    • 1
  1. 1.Institut für Rechtsmedizin der Justus-Liebig-Universität Giessen, Frankfurter Strasse 58, D-35393 Giessen, Germany FAX: +49 (641) 99 4 1429DE

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