Additional primer sets for an amelogenin gene PCR-based DNA-sex test
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When aligning the human amelogenin X (AmelX)-gene with the human amelogenin Y (AmelY)-gene, 19 regions of absolute homology ranging in size from 22 to 80 bp, 5 deletions located on AmelX from 1 to 6 bp and 5 deletions 1–183 bp long located on AmelY can be observed. The regions of absolute homology are used to design primer sets which span deletions of the X- and/ or Y-chromosome. The PCR products generated on the X- and Y-homologues differ in size. New regions of deletions and newly designed primers are described to facilitate the integration of the amelogenin sex test into multiplex PCR reactions.
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