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Population genetic analysis of Chinese Zhuang and Mulao minorities using AGCU-X19 STR kit

  • Cheng Xiao
  • Shuanglin Li
  • Xiaofang Zhang
  • Xingyi Yang
  • Chao LiuEmail author
  • Ling ChenEmail author
Population Data

Abstract

To investigate the genetic variation and forensic efficiency of 19 X-STR loci in the Zhuang and Mulao minorities, we calculated allele frequencies, forensic parameters, and haplotype frequencies of 405 Zhuang and 234 Mulao unrelated healthy individuals from Guangxi Zhuang Autonomous Region of China. Furthermore, we analyzed the population genetic differentiations between the two minorities and other reported Chinese populations.

Keywords

Forensic efficiency Genetic variation X-chromosomal STR Zhuang people Mulao people 

Notes

Acknowledgements

The authors sincerely thank Chunlei Feng and Hong Liu for helping us analyze population data.

Funding information

This work was supported by the Science and Technology Program of Guangzhou, China (No. 201707010486) and Public Research Program of China Institute of Forensic Science (No. 2016CSEEKFKT03).

Compliance with Ethical Standards

Before sampling, we have obtained informed consent letters from all volunteers. This study was approved by the Ethics Committee of Southern Medical University.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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References

  1. 1.
    Szibor R (2007) X-chromosomal markers: past, present and future. Forensic Sci Int Genet 1:93–99.  https://doi.org/10.1016/j.fsigen.2007.03.003 CrossRefGoogle Scholar
  2. 2.
    Yang X, Wu W, Chen L, Liu CH, Zhang XF, Chen L, Feng XL, Wang HJ, Liu C (2016) Development of the 19 X-STR loci multiplex system and genetic analysis of a Zhejiang Han population in China. Electrophoresis 37:2260–2272.  https://doi.org/10.1002/elps.201500540 CrossRefGoogle Scholar
  3. 3.
    Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513Google Scholar
  4. 4.
    Carracedo A, Butler JM, Gusmao L et al (2013) New guidelines for the publication of genetic population data. Forensic Sci Int Genet 7:217–220.  https://doi.org/10.1016/j.fsigen.2013.01.001 CrossRefGoogle Scholar
  5. 5.
    Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567.  https://doi.org/10.1111/j.1755-0998.2010.02847.x CrossRefGoogle Scholar
  6. 6.
    Takezaki N, Nei M, Tamura K (2010) POPTREE2: software for constructing population trees from allele frequency data and computing other population statistics with Windows interface. Mol Biol Evol 27:747–752.  https://doi.org/10.1093/molbev/msp312 CrossRefGoogle Scholar
  7. 7.
    Edelmann J, Hering S, Kuhlisch E, Szibor R (2002) Validation of the STR DXS7424 and the linkage situation on the X-chromosome. Forensic Sci Int 125:217–222CrossRefGoogle Scholar
  8. 8.
    Tomas C, Pereira V, Morling N (2012) Analysis of 12 X-STRs in Greenlanders, Danes and Somalis using Argus X-12. Int J Legal Med 126:121–128.  https://doi.org/10.1007/s00414-011-0609-y CrossRefGoogle Scholar
  9. 9.
    Takezaki N, Nei M (1996) Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics 144:389–399Google Scholar
  10. 10.
    Takezaki N, Nei M (2008) Empirical tests of the reliability of phylogenetic trees constructed with microsatellite DNA. Genetics 178:385–392.  https://doi.org/10.1534/genetics.107.081505 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Forensic MedicineSouthern Medical UniversityGuangzhouChina
  2. 2.Guangzhou Forensic Science InstituteGuangzhouChina

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